Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells

  • Authors:
    • Behjatolah Monzavi-Karbassi
    • R. Jean Hine
    • Joseph S. Stanley
    • Vishnu Prakash Ramani
    • Jaime Carcel-Trullols
    • Tracy L. Whitehead
    • Thomas Kelly
    • Eric R. Siegel
    • Cecile Artaud
    • Saeid Shaaf
    • Rinku Saha
    • Fariba Jousheghany
    • Ronda Henry-Tillman
    • Thomas Kieber-Emmons
  • View Affiliations

  • Published online on: Wednesday, September 1, 2010
  • Pages:615-622 DOI: 10.3892/ijo_00000710
0

Abstract

Aberrant glycosylation is a universal feature of cancer cells, and certain glycan structures are well-known markers for tumor progression. Availability and composition of sugars in the microenvironment may affect cell glycosylation. Recent studies of human breast tumor cell lines indicate their ability to take up and utilize fructose. Here we tested the hypothesis that adding fructose to culture as a carbon source induces phenotypic changes in cultured human breast tumor cells that are associated with metastatic disease. MDA-MB-468 cells were adapted to culture media in which fructose was substituted for glucose. Changes in cell surface glycan structures, expression of genes related to glycan assembly, cytoskeleton F-actin, migration, adhesion and invasion were determined. Cells cultured in fructose expressed distinct cell-surface glycans. The addition of fructose affected sialylation and fucosylation patterns. Fructose feeding also increased binding of leukoagglutinating Phaseolus vulgaris isolectin, suggesting a possible rise in expression of branching β-1, 6 GlcNAc structures. Rhodamine-phalloidin staining revealed an altered F-actin cytoskeletal system. Fructose accelerated cellular migration and increased invasion. These data suggest that changing the carbon source of the less aggressive MDA-MB-468 cell line induced characteristics associated with more aggressive phenotypes. These data could be of fundamental importance due to the markedly increased consumption of sweeteners containing free fructose in recent years, as they suggest that the presence of fructose in nutritional microenvironment of tumor cells may negatively affect the outcome for some breast cancer patients.

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September 2010
Volume 37 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
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APA
Monzavi-Karbassi, B., Hine, R.J., Stanley, J.S., Ramani, V.P., Carcel-Trullols, J., Whitehead, T.L. ... Kieber-Emmons, T. (2010). Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells. International Journal of Oncology, 37, 615-622. http://dx.doi.org/10.3892/ijo_00000710
MLA
Monzavi-Karbassi, B., Hine, R. J., Stanley, J. S., Ramani, V. P., Carcel-Trullols, J., Whitehead, T. L., Kelly, T., Siegel, E. R., Artaud, C., Shaaf, S., Saha, R., Jousheghany, F., Henry-Tillman, R., Kieber-Emmons, T."Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells". International Journal of Oncology 37.3 (2010): 615-622.
Chicago
Monzavi-Karbassi, B., Hine, R. J., Stanley, J. S., Ramani, V. P., Carcel-Trullols, J., Whitehead, T. L., Kelly, T., Siegel, E. R., Artaud, C., Shaaf, S., Saha, R., Jousheghany, F., Henry-Tillman, R., Kieber-Emmons, T."Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells". International Journal of Oncology 37, no. 3 (2010): 615-622. http://dx.doi.org/10.3892/ijo_00000710