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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2010 Volume 37 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2010 Volume 37 Issue 3

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Article

Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines

  • Authors:
    • Weikai Chen
    • Amanda Leiter
    • Dong Yin
    • Muriel Meiring
    • Vernon J. Louw
    • H. Phillip Koeffler

  • View Affiliations / Copyright

    Affiliations: Department of Hematology and Cell Biology, Faculty of Health Sciences, University of the Free State, Bloemfontein 9301, Free State, South Africa
  • Pages: 737-743
    |
    Published online on: September 1, 2010
       https://doi.org/10.3892/ijo_00000723
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Abstract

Cutaneous squamous cell carcinoma (CSCC) is the second most common skin cancer with a substantial risk of metastasis which causes clinical treatment failure. This study investigated the anti-CSCC effects of a triterpenoid compound, Cucurbitacin B (CuB). Dose-response studies showed that CuB inhibited 50% growth (ED50) of the CSCC cell lines (SRB1, SRB12, SCC13, COLO16) in liquid culture at 4x10−7-10−5 M. Soft-agar assays demonstrated that nearly all of the CSCC clonogenic cells were inhibited at 10−7 M CuB. FACS analysis found that the compound (10−7 M, 48 h) caused G2/M arrest. The CSCC cells underwent profound morphologic changes within 60 min after exposure to CuB (10−7 M), rounding up and losing their pseudopodia. CuB (10−7 M) caused prominent multinucleation of the cells after they were pulse-exposed (24 h) to the drug, washed and cultured in normal medium for an additional 24 h. The drug (10−8-10−6 M, 3-24 h) decreased levels of CDC2 and cyclin B1 in SRB1 and SRB12 cell lines as seen by Western blot analysis. Migration of SRB1 and SRB12 cells was inhibited by 10−7 M CuB. Interestingly, CuB synergistically potentiated the anti-proliferative effect of cisplatin in CSCC. In summary, CuB has a prominent anti-proliferative activity on CSCC cells. In vivo studies and clinical trials of this drug should be pursued in CSCC.

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Copy and paste a formatted citation
Spandidos Publications style
Chen W, Leiter A, Yin D, Meiring M, Louw VJ and Koeffler HP: Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines. Int J Oncol 37: 737-743, 2010.
APA
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V.J., & Koeffler, H.P. (2010). Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines. International Journal of Oncology, 37, 737-743. https://doi.org/10.3892/ijo_00000723
MLA
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V. J., Koeffler, H. P."Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines". International Journal of Oncology 37.3 (2010): 737-743.
Chicago
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V. J., Koeffler, H. P."Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines". International Journal of Oncology 37, no. 3 (2010): 737-743. https://doi.org/10.3892/ijo_00000723
Copy and paste a formatted citation
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Spandidos Publications style
Chen W, Leiter A, Yin D, Meiring M, Louw VJ and Koeffler HP: Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines. Int J Oncol 37: 737-743, 2010.
APA
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V.J., & Koeffler, H.P. (2010). Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines. International Journal of Oncology, 37, 737-743. https://doi.org/10.3892/ijo_00000723
MLA
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V. J., Koeffler, H. P."Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines". International Journal of Oncology 37.3 (2010): 737-743.
Chicago
Chen, W., Leiter, A., Yin, D., Meiring, M., Louw, V. J., Koeffler, H. P."Cucurbitacin B inhibits growth, arrests the cell cycle, and potentiates antiproliferative efficacy of cisplatin in cutaneous squamous cell carcinoma cell lines". International Journal of Oncology 37, no. 3 (2010): 737-743. https://doi.org/10.3892/ijo_00000723
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