Differential expression of invasion promoting genes in childhood rhabdomyosarcoma

  • Authors:
    • Sorin Armeanu-Ebinger
    • Michael Bonin
    • Karina Häbig
    • Christopher Poremba
    • Ewa Koscielniak
    • Jan Godzinski
    • Steven W. Warmann
    • Jörg Fuchs
    • Guido Seitz
  • View Affiliations

  • Published online on: January 24, 2011     https://doi.org/10.3892/ijo.2011.921
  • Pages: 993-1000
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Abstract

Expression profiling of tumor tissue allows a systematic search for targeted therapies and offers relevant prognostic information. Molecular studies on rhabdomyosarcoma (RMS) revealed a more differentiated classification than the histological subgrouping into embryonal (RME) and alveolar (RMA) rhabdomyosarcoma, and reflected the chromosomal aberrations found in RMS. We addressed biological processes like cell migration and emerging drug resistance by expression profiling to identify mechanisms of metastasic invasion and differential response to chemotherapy in RMS. Gene expression analysis was performed in 19 RMS samples using the Affymetrix U133 Plus2 array. Validation of target genes was performed by qRT-PCR. Data were analyzed using Pathway analysis software. Involvement of these genes in invasion processes was evaluated in knock-down experiments using specific interference RNA and MatrigelTM invasion assay. In RMA tissues 211 of 534 genes were overexpressed, in RME tissues 323 genes were overexpressed. Pathway analysis software identified a group of genes involved in cell growth, morphology and motility. In patients with distant metastases especially transcription factors such as FOXF1 and LMO4 showed a high expression, which were described as determinants of tumor cell migration. Down-regulation of these factors inhibited the invasion of RMS cells >10-fold. Microarray technology is a powerful method not only to classify RMS samples, but also to identify major regulatory processes. Functional evaluation of LMO4 and FOXF1 identified targets of a molecular network for preventing metastatic invasion in RMS.

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April 2011
Volume 38 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Armeanu-Ebinger S, Bonin M, Häbig K, Poremba C, Koscielniak E, Godzinski J, Warmann SW, Fuchs J and Seitz G: Differential expression of invasion promoting genes in childhood rhabdomyosarcoma. Int J Oncol 38: 993-1000, 2011
APA
Armeanu-Ebinger, S., Bonin, M., Häbig, K., Poremba, C., Koscielniak, E., Godzinski, J. ... Seitz, G. (2011). Differential expression of invasion promoting genes in childhood rhabdomyosarcoma. International Journal of Oncology, 38, 993-1000. https://doi.org/10.3892/ijo.2011.921
MLA
Armeanu-Ebinger, S., Bonin, M., Häbig, K., Poremba, C., Koscielniak, E., Godzinski, J., Warmann, S. W., Fuchs, J., Seitz, G."Differential expression of invasion promoting genes in childhood rhabdomyosarcoma". International Journal of Oncology 38.4 (2011): 993-1000.
Chicago
Armeanu-Ebinger, S., Bonin, M., Häbig, K., Poremba, C., Koscielniak, E., Godzinski, J., Warmann, S. W., Fuchs, J., Seitz, G."Differential expression of invasion promoting genes in childhood rhabdomyosarcoma". International Journal of Oncology 38, no. 4 (2011): 993-1000. https://doi.org/10.3892/ijo.2011.921