Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells

  • Authors:
    • Giovanna Barbieri
    • Emilio Rimini
    • Maria Assunta Costa
  • View Affiliations

  • Published online on: March 29, 2011     https://doi.org/10.3892/ijo.2011.988
  • Pages: 1589-1595
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Abstract

Melanoma cells often display constitutive expression of the major histocompatibility complex (MHC) class II molecules which is associated with a higher metastatic dissemination. The MHC class II molecules during T cell/ professional antigen-presenting cell (APC) interactions are localized, as signalling receptors, into membrane lipid rafts which are thought to be sites of signalling complex assembly. Therefore, with the aim of understanding the molecular mechanisms used by melanoma cells to frustrate an effective anti-tumour response we stimulated a MHC class II consti­tutive expressing melanoma cell line with a specific antibody that mimics the interaction of T-cell receptor (TCR) with class II molecules. In stimulated melanoma cells we showed through Western blotting and immunofluorescence experiments the recruitment of HLA-DR molecules in lipid raft compart­ments as well as the c-Jun NH2-terminal kinase activations as a consequence of the class II engagement. Furthermore, we showed that SDS-stable HLA-DR-peptide complexes are recruited in lipid rafts of stimulated melanoma cells. Therefore, in light of the results reported, our hypothesis is that the redistribution of class II molecules into lipid raft microdomains of stimulated melanoma cells as well as the associated activation of signalling pathways, could be useful for melanoma cells to frustrate an effective anti-tumour response.

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June 2011
Volume 38 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Barbieri G, Rimini E and Costa MA: Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells. Int J Oncol 38: 1589-1595, 2011.
APA
Barbieri, G., Rimini, E., & Costa, M.A. (2011). Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells. International Journal of Oncology, 38, 1589-1595. https://doi.org/10.3892/ijo.2011.988
MLA
Barbieri, G., Rimini, E., Costa, M. A."Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells". International Journal of Oncology 38.6 (2011): 1589-1595.
Chicago
Barbieri, G., Rimini, E., Costa, M. A."Effects of human leukocyte antigen (HLA)-DR engagement on melanoma cells". International Journal of Oncology 38, no. 6 (2011): 1589-1595. https://doi.org/10.3892/ijo.2011.988