Sesamin induces autophagy in colon cancer cells by reducing tyrosine phosphorylation of EphA1 and EphB2

  • Authors:
    • Hiromi Tanabe
    • Kageaki Kuribayashi
    • Naoki Tsuji
    • Maki Tanaka
    • Daisuke Kobayashi
    • Naoki Watanabe
  • View Affiliations

  • Published online on: April 18, 2011     https://doi.org/10.3892/ijo.2011.1011
  • Pages: 33-40
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Abstract

Receptor tyrosine kinase EphB2 and autophagic machinery are known as tumor suppressors; however, the connection remains to be elucidated. Here, we show the link between EphB2 and autophagy. Sesamin, a major lignan in sesame oil, induced autophagy in the human colon cancer cell lines HT29 and LS180, as shown by electron microscopy, as well as Western blotting and immunofluorescence imaging using an anti-LC3 antibody. Receptor tyrosine kinase array analysis revealed that sesamin treatment increased the levels of unphosphorylated -EphA1 and -EphB2 in HT29 cells. Silencing of EphA1 and EphB2 blocked sesamin-induced autophagy as well as sesamin-induced loss of cell viability. These results show that EphA1 and EphB2 play a critical role in this process. The present study reveals a novel function for EphA1 and EphB2 in the induction of autophagy, suggesting a tumor suppressor role for these proteins in colorectal cancer.

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July 2011
Volume 39 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tanabe H, Kuribayashi K, Tsuji N, Tanaka M, Kobayashi D and Watanabe N: Sesamin induces autophagy in colon cancer cells by reducing tyrosine phosphorylation of EphA1 and EphB2. Int J Oncol 39: 33-40, 2011
APA
Tanabe, H., Kuribayashi, K., Tsuji, N., Tanaka, M., Kobayashi, D., & Watanabe, N. (2011). Sesamin induces autophagy in colon cancer cells by reducing tyrosine phosphorylation of EphA1 and EphB2. International Journal of Oncology, 39, 33-40. https://doi.org/10.3892/ijo.2011.1011
MLA
Tanabe, H., Kuribayashi, K., Tsuji, N., Tanaka, M., Kobayashi, D., Watanabe, N."Sesamin induces autophagy in colon cancer cells by reducing tyrosine phosphorylation of EphA1 and EphB2". International Journal of Oncology 39.1 (2011): 33-40.
Chicago
Tanabe, H., Kuribayashi, K., Tsuji, N., Tanaka, M., Kobayashi, D., Watanabe, N."Sesamin induces autophagy in colon cancer cells by reducing tyrosine phosphorylation of EphA1 and EphB2". International Journal of Oncology 39, no. 1 (2011): 33-40. https://doi.org/10.3892/ijo.2011.1011