P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells

  • Authors:
    • Paloma Silva Souza
    • Flavia Cunha Vasconcelos
    • Flaviana Ruade De Souza Reis
    • Gabriela Nestal De Moraes
    • Raquel Ciuvalschi Maia
  • View Affiliations

  • Published online on: June 28, 2011     https://doi.org/10.3892/ijo.2011.1103
  • Pages: 925-933
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Abstract

Overexpression of P-glycoprotein (Pgp/ABCB1) in tumor cells is associated with a classic phenotype of multidrug resistance (MDR). Moreover, some members of the inhibitor of apoptosis protein (IAP) family, such as survivin, contribute to an apoptosis-resistant phenotype, by inhibiting chemotherapy-induced cell death and promoting MDR. By using Western blotting, qRT-PCR, Annexin V and immunofluorescence assays we have demonstrated a relationship between Pgp and survivin in a prior sensitive chronic myeloid leukemia (CML) cell line (K562). A high dose of vincristine induced a concomitant overexpression of Pgp and survivin, which was associated with a low apoptotic index in the K562 cell line. In addition, we observed a cytoplasmic co-localization of Pgp and survivin, suggesting a functional association between these two proteins in apoptosis control by a common mechanism. In summary, our data suggest that Pgp and survivin should be analyzed in aggregate because they may have significant impact on drug resistance in CML cells.

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October 2011
Volume 39 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Souza PS, Vasconcelos FC, De Souza Reis FR, De Moraes GN and Maia RC: P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells. Int J Oncol 39: 925-933, 2011
APA
Souza, P.S., Vasconcelos, F.C., De Souza Reis, F.R., De Moraes, G.N., & Maia, R.C. (2011). P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells. International Journal of Oncology, 39, 925-933. https://doi.org/10.3892/ijo.2011.1103
MLA
Souza, P. S., Vasconcelos, F. C., De Souza Reis, F. R., De Moraes, G. N., Maia, R. C."P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells". International Journal of Oncology 39.4 (2011): 925-933.
Chicago
Souza, P. S., Vasconcelos, F. C., De Souza Reis, F. R., De Moraes, G. N., Maia, R. C."P-glycoprotein and survivin simultaneously regulate vincristine-induced apoptosis in chronic myeloid leukemia cells". International Journal of Oncology 39, no. 4 (2011): 925-933. https://doi.org/10.3892/ijo.2011.1103