Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells

Yeh,Ming-Han; Tsai,Tzung-Chieh; Kuo,Han-Peng; Chang,Nai-Wen; Lee,Miao-Rong; Chung,Jing-Gung; Tsai,Ming-Hsui; Liu,Jah-Yao; Kao,Ming-Ching

doi:10.3892/ijo.2011.1100

November 2011 Volume 39 Issue 5

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November 2011 Volume 39 Issue 5

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Article

Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells

Authors:
- Ming-Han Yeh
- Tzung-Chieh Tsai
- Han-Peng Kuo
- Nai-Wen Chang
- Miao-Rong Lee
- Jing-Gung Chung
- Ming-Hsui Tsai
- Jah-Yao Liu
- Ming-Ching Kao
View Affiliations / Copyright

Affiliations: Graduate Institute of Life Sciences, National Defense Medical Center, No. 161, Section 6, Min-Chuan East Road, Taipei, Taiwan 11490, R.O.C., Department of Obstetrics and Gynecology, Tri-Service General Hospital, No. 325, Section 2, Chenggong Road, Taipei, Taiwan 11490, R.O.C., Department of Biological Science and Technology, College of Life Sciences, China Medical University, 91 Hsueh-Shih Road, Taichung, Taiwan 40402, R.O.C.
Pages: 1221-1231
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Published online on: June 24, 2011

https://doi.org/10.3892/ijo.2011.1100
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Abstract

Oral carcinoma is a serious public health problem and the leading cause of head and neck cancer mortality worldwide. Moreover, oral cancer patients often present symptoms at a late stage and show a high recurrence rate after treatment. Therefore, there is an urgent need to identify novel biomarkers for early diagnosis or clinical oral cancer therapy. In this study, we employed a subset of lentiviral short hairpin RNAs targeted against various kinases and phosphatases, designed by The RNAi Consortium, to screen systemically and in a high-throughput manner for potential growth regulators of oral cancer cells. The screen revealed a total of 50 candidate genes, for which more than 90% of growth inhibition in human oral squamous cancer HSC-3 cells was obtained. Furthermore, bioinformatic analysis of these candidate genes identified transforming growth factor-β receptor type II- and fms-related tyrosine kinase 3-related molecular pathways that are involved in NF-κB-mediated growth of HSC-3 cells. These candidate genes may be potential biomarkers for early diagnosis of oral cancer. In addition, these candidate genes represent potential targets for anticancer drug design helping to develop a personalized treatment to combat oral cancer.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells

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Abstract

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