IMMUNOFLUORESCENT LOCALIZATION AND QUANTITATION OF 3'-AZIDO-2',3'-DIDEOXYTHYMIDINE (AZT) INCORPORATED INTO CHROMOSOMAL DNA OF HUMAN, HAMSTER AND MOUSE-CELL LINES
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- Published online on: January 1, 1994 https://doi.org/10.3892/ijo.4.1.49
- Pages: 49-54
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Abstract
The incorporation of [H-3] AZT into the DNA of cultured hamster (Chinese hamster ovary, CHO) mouse (NIH 3T3) and human (HL60) cells, was studied by radiolabeling, radioimmunoassay, and localization of the drug in chromosomes by immunohistochemistry. Incorporation of AZT into DNA, in cells exposed to 0.8 mM [H-3] AZT for 24 hours, showed values of 0.82 pmol/mug DNA for HL60, 0.84 pmol/mug DNA for CHO and 4.49 pmol/mug for NIH 3T3 cells. Similar results were obtained by radioimmunoassay with an anti-AZT antibody. Indirect immunofluorescence using the same anti-AZT antiserum demonstrated the chromosomal localization of AZT and showed higher concentrations of AZT in the telomeric regions of CHO cell chromosomes.
