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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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April 1994 Volume 4 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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April 1994 Volume 4 Issue 4

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INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE)

  • Authors:
    • K ECKERT
    • T FUHRMANNSELTER
    • M SCHUTT
    • P LANGEN
    • HR MAURER

  • View Affiliations / Copyright

    Affiliations: FREIEN UNIV BERLIN,INST PHARM,PHARMAZEUT BIOCHEM,KELCHSTR 31,D-12169 BERLIN,GERMANY. MAX DELBRUCK CENTRUM MOLEK MED,D-13122 BERLIN,GERMANY.
  • Pages: 853-858
    |
    Published online on: April 1, 1994
       https://doi.org/10.3892/ijo.4.4.853
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Abstract

3'-Deoxy-3'-fluorothymidine (FT), at a concentration of 30 muM, is capable of inducing about 50% of human myeloid K562 cells to differentiate while reducing the growth potential (clonogenicity, growth rate) approximately by one half. For comparison, arabinosylcytosine (Ara C), at 0.1 muM, causes the same percentage of differentiation, but cell proliferation is abolished by >80%. Induction of differentiation is more correlated with the inducer concentration than with incubation periods for both agents. After removal of FT after 5 days of treatment, the cells resume normal growth for the next 5 days. The absolute number of differentiated cells increases within this period. In accordance with their effect on the growth potential, the shift of the colony/cluster ratio towards higher numbers of clusters is greater for Ara C than for FT. Cells treated with either compound are larger than control cells. While in control colonies only very few differentiated cells are found by microscopic inspection, up to 50% of FT treated colony cells are estimated as differentiated. In many clusters and small colonies of Ara C treated cells more than 80% of the cells are benzidine-positive. Loss of clonogenicity of CFU-GM from mouse bone marrow is stronger with Ara C treatment in liquid cell culture for 2 days than with FT treatment in the same concentration range.

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Copy and paste a formatted citation
Spandidos Publications style
ECKERT K, FUHRMANNSELTER T, SCHUTT M, LANGEN P and MAURER H: INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE). Int J Oncol 4: 853-858, 1994.
APA
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., & MAURER, H. (1994). INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE). International Journal of Oncology, 4, 853-858. https://doi.org/10.3892/ijo.4.4.853
MLA
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., MAURER, H."INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE)". International Journal of Oncology 4.4 (1994): 853-858.
Chicago
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., MAURER, H."INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE)". International Journal of Oncology 4, no. 4 (1994): 853-858. https://doi.org/10.3892/ijo.4.4.853
Copy and paste a formatted citation
x
Spandidos Publications style
ECKERT K, FUHRMANNSELTER T, SCHUTT M, LANGEN P and MAURER H: INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE). Int J Oncol 4: 853-858, 1994.
APA
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., & MAURER, H. (1994). INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE). International Journal of Oncology, 4, 853-858. https://doi.org/10.3892/ijo.4.4.853
MLA
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., MAURER, H."INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE)". International Journal of Oncology 4.4 (1994): 853-858.
Chicago
ECKERT, K., FUHRMANNSELTER, T., SCHUTT, M., LANGEN, P., MAURER, H."INDUCTION OF DIFFERENTIATION OF HUMAN MYELOID LEUKEMIC K562 CELLS BY 3'-DEOXY-3'-FLUOROTHYMIDINE (COMPARISON WITH ARABINOSYLCYTOSINE)". International Journal of Oncology 4, no. 4 (1994): 853-858. https://doi.org/10.3892/ijo.4.4.853
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