SENSITIVITY OF DRUG-RESISTANT B-CELL LINES FROM AIDS-RELATED NON-HODGKINS-LYMPHOMA TO NEWLY SYNTHESIZED PODOPHYLLOTOXIN DERIVATIVES AND AZA-ALKYLLYSOPHOSPHOLIPIDS - ENHANCED SENSITIZATION BY PRETREATMENT WITH INTERFERON-GAMMA

  • Authors:
    • A DEMIDEM
    • C BROQUET
    • JM MENCIAHUERTA
    • SZ SALAHUDDIN
    • T LAM
    • RS KHAN
    • P BRAQUET
    • AM LEVINE
    • B BONAVIDA
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1203
  • Pages: 1203-1209
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Abstract

Non-Hodgkin's lymphoma B cells (NHL-B) often become refractory to conventional chemotherapeutic drugs. The present study investigated the sensitivity of three AIDS-derived NHL-B cell lines to newly synthesized azaalkyllysophospholipids (AALP) and podophyllotoxin derivatives. All three cell lines were sensitive to 5 AALP derivatives and toxicity was detectable at 24 h of culture and was increased at 48 and 72 h of incubation. Toxicity was concentration-dependent and die extent of cytotoxicity varied slightly from one cell line to another. These cell lines were less sensitive to the podophyllotoxin derivatives VP-16, BDPTN and BEPT than to die AALP. This was shown by the extent of cytotoxicity calculated on a molar basis and by the delayed kinetics of lysis. Pretreatment of the tumor cells with IFN-gamma stimulated cell proliferation. IFN-gamma treated tumor cells were also tested for their sensitivity to the various cytotoxic agents. In all cases, the sensitivity of the IFN-gamma pretreated cell lines to the podophyllotoxin derivatives and AALP was significantly enhanced. These findings demonstrate that drug resistant NHL-B cells are sensitive to a new family of AALP and podophyllotoxin derivatives. Further, the sensitivity of the tumor cells was enhanced by treatment with IFN-gamma. The potential clinical use of these new cytotoxic agents to overcome resistance of AIDS-related cell lymphomas is discussed.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
DEMIDEM A, BROQUET C, MENCIAHUERTA J, SALAHUDDIN S, LAM T, KHAN R, BRAQUET P, LEVINE A and BONAVIDA B: SENSITIVITY OF DRUG-RESISTANT B-CELL LINES FROM AIDS-RELATED NON-HODGKINS-LYMPHOMA TO NEWLY SYNTHESIZED PODOPHYLLOTOXIN DERIVATIVES AND AZA-ALKYLLYSOPHOSPHOLIPIDS - ENHANCED SENSITIZATION BY PRETREATMENT WITH INTERFERON-GAMMA. Int J Oncol 4: 1203-1209, 1994.
APA
DEMIDEM, A., BROQUET, C., MENCIAHUERTA, J., SALAHUDDIN, S., LAM, T., KHAN, R. ... BONAVIDA, B. (1994). SENSITIVITY OF DRUG-RESISTANT B-CELL LINES FROM AIDS-RELATED NON-HODGKINS-LYMPHOMA TO NEWLY SYNTHESIZED PODOPHYLLOTOXIN DERIVATIVES AND AZA-ALKYLLYSOPHOSPHOLIPIDS - ENHANCED SENSITIZATION BY PRETREATMENT WITH INTERFERON-GAMMA. International Journal of Oncology, 4, 1203-1209. https://doi.org/10.3892/ijo.4.6.1203
MLA
DEMIDEM, A., BROQUET, C., MENCIAHUERTA, J., SALAHUDDIN, S., LAM, T., KHAN, R., BRAQUET, P., LEVINE, A., BONAVIDA, B."SENSITIVITY OF DRUG-RESISTANT B-CELL LINES FROM AIDS-RELATED NON-HODGKINS-LYMPHOMA TO NEWLY SYNTHESIZED PODOPHYLLOTOXIN DERIVATIVES AND AZA-ALKYLLYSOPHOSPHOLIPIDS - ENHANCED SENSITIZATION BY PRETREATMENT WITH INTERFERON-GAMMA". International Journal of Oncology 4.6 (1994): 1203-1209.
Chicago
DEMIDEM, A., BROQUET, C., MENCIAHUERTA, J., SALAHUDDIN, S., LAM, T., KHAN, R., BRAQUET, P., LEVINE, A., BONAVIDA, B."SENSITIVITY OF DRUG-RESISTANT B-CELL LINES FROM AIDS-RELATED NON-HODGKINS-LYMPHOMA TO NEWLY SYNTHESIZED PODOPHYLLOTOXIN DERIVATIVES AND AZA-ALKYLLYSOPHOSPHOLIPIDS - ENHANCED SENSITIZATION BY PRETREATMENT WITH INTERFERON-GAMMA". International Journal of Oncology 4, no. 6 (1994): 1203-1209. https://doi.org/10.3892/ijo.4.6.1203