BCL-2 ONCOGENE BLOCKS DIFFERENTIATION AND EXTENDS VIABILITY BUT DOES NOT IMMORTALIZE NORMAL HUMAN KERATINOCYTES

  • Authors:
    • A NATARAJ
    • S PATHAK
    • VL HOPWOOD
    • TJ MCDONNELL
    • HN ANANTHASWAMY
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1211
  • Pages: 1211-1218
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Abstract

To ascertain whether the bcl-2 oncogene plays a role in the initial stages of skin carcinogenesis by preventing differentiation of epidermal keratinocytes, we transfected primary human keratinocytes with the human bcl-2 gene and then determined whether these transfectants escape high calcium- and serum-induced differentiation. We found that the bcl-2 oncogene blocked differentiation and extended the life span of human keratinocytes in culture by over 24 weeks compared with cells transfected with pZip-neo DNA, which only grew for 5 weeks in culture. Keratinocytes transfected with the bcl-2 oncogene exhibited apoptotic bodies and telomere-telomere association between chromosomes toward the end of their life-span. These results suggest that die bcl-2 oncogene may be necessary but not sufficient for the immortalization of human keratinocytes.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
NATARAJ A, PATHAK S, HOPWOOD V, MCDONNELL T and ANANTHASWAMY H: BCL-2 ONCOGENE BLOCKS DIFFERENTIATION AND EXTENDS VIABILITY BUT DOES NOT IMMORTALIZE NORMAL HUMAN KERATINOCYTES. Int J Oncol 4: 1211-1218, 1994
APA
NATARAJ, A., PATHAK, S., HOPWOOD, V., MCDONNELL, T., & ANANTHASWAMY, H. (1994). BCL-2 ONCOGENE BLOCKS DIFFERENTIATION AND EXTENDS VIABILITY BUT DOES NOT IMMORTALIZE NORMAL HUMAN KERATINOCYTES. International Journal of Oncology, 4, 1211-1218. https://doi.org/10.3892/ijo.4.6.1211
MLA
NATARAJ, A., PATHAK, S., HOPWOOD, V., MCDONNELL, T., ANANTHASWAMY, H."BCL-2 ONCOGENE BLOCKS DIFFERENTIATION AND EXTENDS VIABILITY BUT DOES NOT IMMORTALIZE NORMAL HUMAN KERATINOCYTES". International Journal of Oncology 4.6 (1994): 1211-1218.
Chicago
NATARAJ, A., PATHAK, S., HOPWOOD, V., MCDONNELL, T., ANANTHASWAMY, H."BCL-2 ONCOGENE BLOCKS DIFFERENTIATION AND EXTENDS VIABILITY BUT DOES NOT IMMORTALIZE NORMAL HUMAN KERATINOCYTES". International Journal of Oncology 4, no. 6 (1994): 1211-1218. https://doi.org/10.3892/ijo.4.6.1211