THE EFFECT OF FGF-3 INT-2 ON GROWTH AND TRANSFORMATION OF MCF-10A NORMAL HUMAN MAMMARY EPITHELIAL-CELLS IS DISTINCT FROM FGF-1 AND FGF-2

  • Authors:
    • F BASOLO
    • T VENESIO
    • S CALVO
    • L FIORE
    • G FONTANINI
    • F CIARDIELLO
    • A TONIOLO
    • DS LISCIA
    • GR MERLO
  • View Affiliations

  • Published online on: June 1, 1994     https://doi.org/10.3892/ijo.4.6.1365
  • Pages: 1365-1370
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Abstract

Fibroblast growth factors (FGFs) are related polypeptides with mitogenic activity on cells of mesodermal and neuroectodermal origin. The Fgf-3 gene shares high homology with FGF-2 and its protein product can substitute FGF-2 as a growth factor. Other observations, however, indicate that Fgf-3 has specialized functions. We have investigated the effect of the expression and secretion of Fgf-3 on the growth and transformation of the human breast epithelial cell line MCF-10A. Overexpression of Fgf-3 stimulates proliferation of these cells in serum-free medium and induces anchorage-independent colony formation in soft agar. In contrast, these effects were not observed with purified FGF-1 and FGF-2 on either the parental or the Fgf-3-MCF-10A cells. Thus, Fgf-3 is distinct from FGF-1 and FGF-2 for its ability to induce cell proliferation and transformation of MCF-10A cells. This difference could be due, at least in part, to the expression of a specific set of FGF receptors with higher affinity for FGF-3 than FGF-1 or FGF-2.

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June 1994
Volume 4 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
BASOLO F, VENESIO T, CALVO S, FIORE L, FONTANINI G, CIARDIELLO F, TONIOLO A, LISCIA D and MERLO G: THE EFFECT OF FGF-3 INT-2 ON GROWTH AND TRANSFORMATION OF MCF-10A NORMAL HUMAN MAMMARY EPITHELIAL-CELLS IS DISTINCT FROM FGF-1 AND FGF-2. Int J Oncol 4: 1365-1370, 1994.
APA
BASOLO, F., VENESIO, T., CALVO, S., FIORE, L., FONTANINI, G., CIARDIELLO, F. ... MERLO, G. (1994). THE EFFECT OF FGF-3 INT-2 ON GROWTH AND TRANSFORMATION OF MCF-10A NORMAL HUMAN MAMMARY EPITHELIAL-CELLS IS DISTINCT FROM FGF-1 AND FGF-2. International Journal of Oncology, 4, 1365-1370. https://doi.org/10.3892/ijo.4.6.1365
MLA
BASOLO, F., VENESIO, T., CALVO, S., FIORE, L., FONTANINI, G., CIARDIELLO, F., TONIOLO, A., LISCIA, D., MERLO, G."THE EFFECT OF FGF-3 INT-2 ON GROWTH AND TRANSFORMATION OF MCF-10A NORMAL HUMAN MAMMARY EPITHELIAL-CELLS IS DISTINCT FROM FGF-1 AND FGF-2". International Journal of Oncology 4.6 (1994): 1365-1370.
Chicago
BASOLO, F., VENESIO, T., CALVO, S., FIORE, L., FONTANINI, G., CIARDIELLO, F., TONIOLO, A., LISCIA, D., MERLO, G."THE EFFECT OF FGF-3 INT-2 ON GROWTH AND TRANSFORMATION OF MCF-10A NORMAL HUMAN MAMMARY EPITHELIAL-CELLS IS DISTINCT FROM FGF-1 AND FGF-2". International Journal of Oncology 4, no. 6 (1994): 1365-1370. https://doi.org/10.3892/ijo.4.6.1365