Downregulation of microRNA-431 by human interferon-β inhibits viability of medulloblastoma and glioblastoma cells via upregulation of SOCS6

  • Authors:
    • Takeshi Tanaka
    • Makoto Arai
    • Xia Jiang
    • Shigeru Sugaya
    • Tatsuo Kanda
    • Katsunori Fujii
    • Kazuko Kita
    • Katsuo Sugita
    • Fumio Imazeki
    • Toshiyuki Miyashita
    • Atsushi Kaneda
    • Osamu Yokosuka
  • View Affiliations

  • Published online on: February 28, 2014     https://doi.org/10.3892/ijo.2014.2317
  • Pages: 1685-1690
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Abstract

miRNAs are small non-coding RNAs that inhibit gene expression by cleaving or hindering the translation of target mRNAs. In this study, we focused on miR-431, which mediated inhibition of cell viability by human interferon-β (HuIFN-β). We aimed to demonstrate an antineoplastic effect of HuIFN-β via miR-431 expression against medulloblastoma and glioblastoma, because HuIFN-β is frequently used in adjuvant therapy of these tumors. Addition of HuIFN-β to medulloblastoma and glioblastoma cells reduced viability, significantly decreased miR-431 expression, upregulated expression of SOCS6 (putative miR-431 target genes) and inhibited Janus kinase (JAK) 1 and signal transducer and activator of transcription (STAT) 2. The mitogen-activated protein kinase (MAPK) pathway, but not the phosphoinositide 3-kinase (PI3K)-Akt pathway, was downregulated in medulloblastoma cells, whereas the PI3K-Akt pathway, but not the MAPK pathway, was downregulated in glioblastoma cells. Addition of HuIFN-β and transient transfection with miR-431 to medulloblastoma and glioblastoma cells did not reduce viability, downregulated expression of SOCS6, and concomitantly activated the JAK1 and STAT2. We propose that, in medulloblastoma and glioblastoma cells, HuIFN-β decreases miR-431 expression and upregulates SOCS6 expression, and consequently inhibit cell proliferation by suppressing the JAK-STAT signaling pathway.
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May-2014
Volume 44 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tanaka T, Arai M, Jiang X, Sugaya S, Kanda T, Fujii K, Kita K, Sugita K, Imazeki F, Miyashita T, Miyashita T, et al: Downregulation of microRNA-431 by human interferon-β inhibits viability of medulloblastoma and glioblastoma cells via upregulation of SOCS6. Int J Oncol 44: 1685-1690, 2014.
APA
Tanaka, T., Arai, M., Jiang, X., Sugaya, S., Kanda, T., Fujii, K. ... Yokosuka, O. (2014). Downregulation of microRNA-431 by human interferon-β inhibits viability of medulloblastoma and glioblastoma cells via upregulation of SOCS6. International Journal of Oncology, 44, 1685-1690. https://doi.org/10.3892/ijo.2014.2317
MLA
Tanaka, T., Arai, M., Jiang, X., Sugaya, S., Kanda, T., Fujii, K., Kita, K., Sugita, K., Imazeki, F., Miyashita, T., Kaneda, A., Yokosuka, O."Downregulation of microRNA-431 by human interferon-β inhibits viability of medulloblastoma and glioblastoma cells via upregulation of SOCS6". International Journal of Oncology 44.5 (2014): 1685-1690.
Chicago
Tanaka, T., Arai, M., Jiang, X., Sugaya, S., Kanda, T., Fujii, K., Kita, K., Sugita, K., Imazeki, F., Miyashita, T., Kaneda, A., Yokosuka, O."Downregulation of microRNA-431 by human interferon-β inhibits viability of medulloblastoma and glioblastoma cells via upregulation of SOCS6". International Journal of Oncology 44, no. 5 (2014): 1685-1690. https://doi.org/10.3892/ijo.2014.2317