Association of EMP1 with gastric carcinoma invasion, survival and prognosis

  • Authors:
    • Guogui Sun
    • Gang Zhao
    • Yifang Lu
    • Yadi Wang
    • Congrong Yang
  • View Affiliations

  • Published online on: June 10, 2014     https://doi.org/10.3892/ijo.2014.2488
  • Pages: 1091-1098
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Abstract

The aim of this study was to determine the expression and function of epithelial membrane protein 1 (EMP1) in gastric carcinoma. Gastric samples were taken from cancer lesions and adjacent normal tissue in gastric cancer patients immediately after endoscopic biopsy. A portion of the sample was either fixed in 4% paraformaldehyde and embedded in paraffin for immunohistochemistry or stored in liquid nitrogen for western blotting. In order to determine protein expression of EMP1 in gastric cancer (n=65) and normal tissue (n=27), semi-quantitative immunohistochemistry and western blotting were utilized. For in vitro studies, the human gastric cancer cell line SGC-7901 was maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum. Recombinant lentivirus mediated overexpression of EMP1 in SGC-7901 cells was quantified with quantitative polymerase chain reaction (qPCR) and western blotting. Control SGC-7901 cells were transfected with an empty vector. To further study the effect of EMP1 overexpression in SGC-7901 cells, cell prolifer­ation, cell apoptosis and migration and invasion assays were conducted. The expression of EMP1 was significantly lower in gastric cancer tissue compared to normal tissue using both immunohistochemistry (41.5 vs. 70.4% of tissues, P<0.05) and western blotting (0.153±0.012 vs. 0.626±0.058, P<0.05). Decreased expression of EMP1 was significantly correlated with tumor invasion, lymph node metastasis, clinical stage and histological grade of patients with gastric cancer (P<0.05). According to Kaplan-Meier analysis, low EMP1 expression correlated significantly with poor overall 5‑year survival (47.4 vs. 70.3% survival, P<0.05). SGC-7901 cells transfected with EMP1 had a lower survival fraction, higher cell apoptosis (13.2±1.5% vs. 2.2±0.5%, P<0.05), significant decrease in migration and invasion (157.0±16.0 and 112.0±12.0, respectively vs. 243.0±21.0 and 203.0±19.0, respectively, P<0.05), higher caspase-9 (0.501±0.050 vs. 0.114±0.010, P<0.05) and lower VEGFC protein expression 0.135±0.011 vs. 0.619±0.074, P<0.05) relative to cells not transfected with EMP1. Low EMP1 expression in gastric cancer is associated with increased disease severity, suggesting that EMP1 may be a negative regulator of gastric cancer.
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September-2014
Volume 45 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sun G, Zhao G, Lu Y, Wang Y and Yang C: Association of EMP1 with gastric carcinoma invasion, survival and prognosis. Int J Oncol 45: 1091-1098, 2014
APA
Sun, G., Zhao, G., Lu, Y., Wang, Y., & Yang, C. (2014). Association of EMP1 with gastric carcinoma invasion, survival and prognosis. International Journal of Oncology, 45, 1091-1098. https://doi.org/10.3892/ijo.2014.2488
MLA
Sun, G., Zhao, G., Lu, Y., Wang, Y., Yang, C."Association of EMP1 with gastric carcinoma invasion, survival and prognosis". International Journal of Oncology 45.3 (2014): 1091-1098.
Chicago
Sun, G., Zhao, G., Lu, Y., Wang, Y., Yang, C."Association of EMP1 with gastric carcinoma invasion, survival and prognosis". International Journal of Oncology 45, no. 3 (2014): 1091-1098. https://doi.org/10.3892/ijo.2014.2488