The co-localization of HBx and COXIII upregulates COX-2 promoting HepG2 cell growth

Zheng,Bi-Yun; Fang,Xue-Fen; Zou,Lai-Yu; Huang,Yue-Hong; Chen,Zhi-Xin; Li,Dan; Zhou,Lin-Ying; Chen,Hao; Wang,Xiao-Zhong

doi:10.3892/ijo.2014.2499

The co-localization of HBx and COXIII upregulates COX-2 promoting HepG2 cell growth

Authors:
- Bi-Yun Zheng
- Xue-Fen Fang
- Lai-Yu Zou
- Yue-Hong Huang
- Zhi-Xin Chen
- Dan Li
- Lin-Ying Zhou
- Hao Chen
- Xiao-Zhong Wang
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Affiliations: Graduate School, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Department of Gastroenterology, Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Department of Infection, Union Hospital of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Laboratory of Electron Microscopy, Fujian Medical University, Fuzhou, Fujian 350001, P.R. China, Department of Gastroenterology, The Third Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350108, P.R. China
Published online on: June 16, 2014 https://doi.org/10.3892/ijo.2014.2499
Pages: 1143-1150

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Abstract

HBx is a multifunctional regulator that interacts with host factors to contribute to the development of hepatocellular carcinoma. In this study, to explore the co-localization of HBx and COXIII in HepG2 cells and to investigate the molecular mechanism of HBx in HepG2 cell growth promotion, we first constructed a HepG2 cell line stably expressing the HBx gene in vitro by lentivirus vectors. In addition, we found that HBx co-localized with the inner mitochondrial protein, COXIII, in HepG2 cells by confocal laser scanning microscopy. It led to changes of mitochondrial biogenesis and morphology, including upregulation of COXIII protein expression, increased cytochrome c oxidase activity and higher mitochondrial membrane potential. The upregulation of COX-2 caused by HBx through generation of mitochondrial reactive oxygen species promoted cell growth. Thus, we conclude that co-localization of HBx and COXIII leads to upregulation of COX-2 that promotes HepG2 cell growth. Such a mechanism provides deeper insights into the molecular mechanism of HBV-associated hepatocellular carcinoma.

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