Anti-inflammatory effects of betaine on AOM/DSS‑induced colon tumorigenesis in ICR male mice

Kim,Dong  Hwan; Sung,Bokyung; Kang,Yong  Jung; Jang,Jung  Yoon; Hwang,Seong  Yeon; Lee,Yujin; Kim,Minjung; Im,Eunok; Yoon,Jeong-Hyun; Kim,Cheol  Min; Chung,Hae  Young; Kim,Nam  Deuk

doi:10.3892/ijo.2014.2515

Anti-inflammatory effects of betaine on AOM/DSS‑induced colon tumorigenesis in ICR male mice

Authors:
- Dong Hwan Kim
- Bokyung Sung
- Yong Jung Kang
- Jung Yoon Jang
- Seong Yeon Hwang
- Yujin Lee
- Minjung Kim
- Eunok Im
- Jeong-Hyun Yoon
- Cheol Min Kim
- Hae Young Chung
- Nam Deuk Kim
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Affiliations: Department of Pharmacy, Molecular Inflammation Research Center for Aging Intervention, Pusan National University, Busan 609‑735, Republic of Korea, Research Center for Anti‑Aging Technology Development, Pusan National University, Busan 609‑735, Republic of Korea
Published online on: June 24, 2014 https://doi.org/10.3892/ijo.2014.2515
Pages: 1250-1256

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Abstract

Betaine is an important human nutrient obtained from various foods and studies in animals and humans have provided results suggesting their pathogenesis of various chronic diseases and points to a role in risk assessment and disease prevention. However, the molecular mechanisms of its activity remain poorly understood and warrant further investigation. This study was performed to investigate the anti-inflammation and tumor preventing capacity of betaine on colitis-associated cancer in mice. In in vivo experiments, we induced colon tumors in mice by azoxymethane (AOM) and dextran sulfate sodium (DSS) and evaluated the effects of betaine on tumor growth. Administration with betaine significantly decreased the incidence of tumor formation with downregulation of inflammation. Treatment with betaine inhibited ROS generation and GSSG concentration in colonic mucosa. Based on the qPCR data, administration of betaine inhibited inflammatory cytokines such TNF-α, IL-6, iNOS and COX-2. In in vitro experiments, LPS-induced NF-κB and inflammatory-related cytokines were inhibited by betaine treatment in RAW 264.7 murine macrophage cells. Our findings suggest that betaine is one of the candidates for the prevention of inflammation-associated colon carcinogenesis.

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