miR-126 inhibits growth of SGC-7901 cells by synergistically targeting the oncogenes PI3KR2 and Crk, and the tumor suppressor PLK2

Liu,Li  Ying; Wang,Wei; Zhao,Lin  Yu; Guo,Bo; Yang, Juan; Zhao,Xiao  Ge; Hou,Ni; Ni,Lei; Wang,Ai  Ying; Song,Tu  Sheng; Huang,Chen; Xu,Ji  Ru

doi:10.3892/ijo.2014.2516

miR-126 inhibits growth of SGC-7901 cells by synergistically targeting the oncogenes PI3KR2 and Crk, and the tumor suppressor PLK2

Authors:
- Li Ying Liu
- Wei Wang
- Lin Yu Zhao
- Bo Guo
- Juan Yang
- Xiao Ge Zhao
- Ni Hou
- Lei Ni
- Ai Ying Wang
- Tu Sheng Song
- Chen Huang
- Ji Ru Xu
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Affiliations: The Center Laboratory for Biomedical Research, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710004, P.R. China, Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Genetics and Cell Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China
Published online on: June 24, 2014 https://doi.org/10.3892/ijo.2014.2516
Pages: 1257-1265

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Abstract

MicroRNA (miRNA)-126 (miR-126) was reported to be downregulated and to act as a tumor suppressor in cancers of the lung, cervix, bladder and prostate. However, the functions of miR-126 in gastric cancer appear to be diverse and are largely unknown. MiR-126 was reported to act as a tumor suppressor by targeting the Crk gene, or as an oncogene by targeting the SOX2 gene in gastric cancer. We identified that the expression of miR-126 was decreased in gastric cancer cell lines and tissues. PLK2, a tumor suppressor gene, was directly regulated by miR-126 in SGC-7901 cells. Overexpression of miR-126 not only suppressed the growth and clone formation of SGC-7901 cells, but also induced apoptosis in vitro, whereas inhibition of miR-126 slightly promoted SGC-7901 cell proliferation. The cell cycle was not affected by miR-126. Moreover, miR-126 suppressed tumor growth in vivo in a xenograft model. PLK2, PI3KR2 and Crk were regulated by miR-126 in SGC-7901 cells. We infer that the functions of miR-126 in gastric cancer depend on synergistic targeting balance between oncogenes and anti-oncogenes. Our study indicates that miR-126 is a tumor suppressor, which in the future may become a therapeutic target for gastric cancer.

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