Open Access

Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro

  • Authors:
    • Xiaokang Zhou
    • Danqing Huang
    • Zhongxiu Xue
    • Xiuhui Xu
    • Kai Wang
    • Yao Sun
    • Feiwu Kang
  • View Affiliations

  • Published online on: March 26, 2015     https://doi.org/10.3892/ijo.2015.2934
  • Pages: 2346-2354
  • Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Hypoxia-inducible factor-1α (HIF-1α) is a key regulator for tumor cells and tissues to adapt to hypoxic condition. Suppressing the expression of HIF-1α is important to evaluate its effect on cancer cells. This study was carried out to analyze the effect of HIF-1α on the biological activation of human tongue squamous cell carcinoma (TSCC) SCC-15 cells. In this experiment, deferoxamine mesylate (DFO) was used to induce hypoxic condition. HIF-1α gene was suppressed by lentiviral vector. The effect of the level of HIF-1α expression was tested on the proliferation, cell cycle, cell apoptosis and cell invasion of SCC-15 cells. We demonstrated that SCC-15 cells showed a more aggressive phenotype after treated with DFO. Additionally, DFO was able to induce the expression of HIF-1α protein. Lentiviral vector can effectively inhibit HIF-1α expression on mRNA and protein level. Under normoxic or hypoxic conditions, downregulation of HIF-1α for SCC-15 cells induced cell apoptosis and inhibited growth and invasion. These results showed that suppressing the expression of HIF-1α inhibited the aggressive potential of SCC-15 cells under normoxic and hypoxic condition. Thus, finding an effective and safe pathway to inhibit the expression of HIF-1α can help us to improve the survival rate of human TSCC patients.
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June-2015
Volume 46 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhou X, Huang D, Xue Z, Xu X, Wang K, Sun Y and Kang F: Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro. Int J Oncol 46: 2346-2354, 2015.
APA
Zhou, X., Huang, D., Xue, Z., Xu, X., Wang, K., Sun, Y., & Kang, F. (2015). Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro. International Journal of Oncology, 46, 2346-2354. https://doi.org/10.3892/ijo.2015.2934
MLA
Zhou, X., Huang, D., Xue, Z., Xu, X., Wang, K., Sun, Y., Kang, F."Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro". International Journal of Oncology 46.6 (2015): 2346-2354.
Chicago
Zhou, X., Huang, D., Xue, Z., Xu, X., Wang, K., Sun, Y., Kang, F."Effect of HIF-1α on biological activation of human tongue squamous cell carcinoma SCC-15 cells in vitro". International Journal of Oncology 46, no. 6 (2015): 2346-2354. https://doi.org/10.3892/ijo.2015.2934