Kahweol induces apoptosis by suppressing BTF3 expression through the ERK signaling pathway in non-small cell lung cancer cells

Jeon,Young-Joo; Bang,Woong; Cho,Jin Hyoung; Lee,Ra Ham; Kim,Seok-Ho; Kim,Minseok S.; Park,Seon-Min; Shin,Jae-Cheon; Chung,Hak-Jae; Oh,Keon Bong; Seo,Jae-Min; Ko,Sungho; Shim,Jung-Hyun; Chae,Jung-Il

doi:10.3892/ijo.2016.3727

Kahweol induces apoptosis by suppressing BTF3 expression through the ERK signaling pathway in non-small cell lung cancer cells

Authors:
- Young-Joo Jeon
- Woong Bang
- Jin Hyoung Cho
- Ra Ham Lee
- Seok-Ho Kim
- Minseok S. Kim
- Seon-Min Park
- Jae-Cheon Shin
- Hak-Jae Chung
- Keon Bong Oh
- Jae-Min Seo
- Sungho Ko
- Jung-Hyun Shim
- Jung-Il Chae
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Affiliations: Department of Dental Pharmacology, School of Dentistry, BK21 Plus, Chonbuk National University, Jeonju, Jeolla 561‑756, Republic of Korea, Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Republic of Korea, Department of Biomedical Engineering, Konyang University, Seo, Daejeon, Republic of Korea, Pohang Center for Evaluation of Biomaterials, Pohang, Gyeongsang 790‑834, Republic of Korea, Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Suwon, Gyeonggi, Republic of Korea, Cluster for Craniofacial Development and Regeneration Research, Institute of Oral Biosciences and School of Dentistry, Chonbuk National University, Jeonju, Jeolla, Republic of Korea, Department of Applied Bioscience, CHA University, Seongnam, Gyeonggi 463-836, Republic of Korea, Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Mokpo, Jeolla 534-729, Republic of Korea
Published online on: October 12, 2016 https://doi.org/10.3892/ijo.2016.3727
Pages: 2294-2302

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Abstract

Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it has apoptotic effect in non-small cell lung cancer (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC cell lines, NCI-H358 and NCI‑H1299, with different concentrations of kahweol and used the MTS assay, DAPI staining, propidium iodide staining, Annexin V staining, immunocytochemical test, and western blot analysis to characterize this molecule and the signaling pathway underlying its effects. The kahweol-treated cells showed significantly decreased cell viability, increased nuclear condensation, and an increased number of Annexin V-positive NSCLC cells. Suppression of basic transcription factor 3 (BTF3) was followed by apoptosis induced by kahweol via the ERK-mediated signaling pathway in a dose- and time-dependent manner. In addition, kahweol modulated the protein expression of BTF3 genes involved in cell-cycle regulation and apoptosis-related proteins, resulting in apoptotic cell death. Our results collectively indicated that kahweol inhibited the proliferation of NSCLC cells through ERK-mediated signaling pathways and the downregulation of BTF3.

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