INDUCTION BY HUMAN INTERFERON AND MURINE H-2L(D) GENE OF THE SURFACE EXPRESSION OF ENDOGENOUS HLA CLASS-I FREE HEAVY-CHAINS IN HCT HUMAN COLON-CARCINOMA CELLS, DEFICIENT IN BETA-2-MICROGLOBULIN

  • Authors:
    • MM SIMILE
    • L CALORINI
    • MS RYAN
    • S GATTONICELLI
  • View Affiliations

  • Published online on: August 1, 1994     https://doi.org/10.3892/ijo.5.2.181
  • Pages: 181-188
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Abstract

The colon cancer cell line HCT fails to express HLA class I antigens on the cell surface as a consequence of two independent mutations occurring in both copies of the beta2-microglobulin (beta2-mu) gene. Restoration of HLA class I antigen expression in HCT cells transfected with the wild-type human beta2-mu gene is accompanied by the expression of beta2-mu-free HLA class I heavy chains on the cell surface. HCT cells expressing a transfected H-2L(d) gene (HCT-L(d) transfectants) exhibited high levels of H-2L(d) heavy chains on the cell surface, following treatment with human interferon a (IFN-alpha). IFN-treated HCT-L(d) transfectants also expressed high levels of endogenous beta2-g-free HLA class I heavy chains on the cell surface. Incubation of HCT-L(d) transfectants at 26-degrees-C for 24 hours is associated with a striking increase in the surface expression of H-2L(d) heavy chains (comparable to that observed at 37-degrees-C in the presence of IFN), without a concomitant increase in the surface expression of beta2-mu-free HLA class I heavy chains, suggesting that IFN and low temperature act by different mechanisms. These results also indicate that human IFN and H-2L(d) heavy chain can act synergistically in inducing the surface expression of endogenous beta2-mu-free HLA class I heavy chains by HCT cells. The combination of 26-degrees-C incubation and IFN treatment results in the surface expression of beta2-mu-free HLA class I heavy chains in HCT-neo transfectants, suggesting that IFN plays a crucial role in allowing the surface expression of endogenous HLA class I heavy chains in beta2-mu-negative HCT cells.

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August 1994
Volume 5 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
SIMILE M, CALORINI L, RYAN M and GATTONICELLI S: INDUCTION BY HUMAN INTERFERON AND MURINE H-2L(D) GENE OF THE SURFACE EXPRESSION OF ENDOGENOUS HLA CLASS-I FREE HEAVY-CHAINS IN HCT HUMAN COLON-CARCINOMA CELLS, DEFICIENT IN BETA-2-MICROGLOBULIN. Int J Oncol 5: 181-188, 1994.
APA
SIMILE, M., CALORINI, L., RYAN, M., & GATTONICELLI, S. (1994). INDUCTION BY HUMAN INTERFERON AND MURINE H-2L(D) GENE OF THE SURFACE EXPRESSION OF ENDOGENOUS HLA CLASS-I FREE HEAVY-CHAINS IN HCT HUMAN COLON-CARCINOMA CELLS, DEFICIENT IN BETA-2-MICROGLOBULIN. International Journal of Oncology, 5, 181-188. https://doi.org/10.3892/ijo.5.2.181
MLA
SIMILE, M., CALORINI, L., RYAN, M., GATTONICELLI, S."INDUCTION BY HUMAN INTERFERON AND MURINE H-2L(D) GENE OF THE SURFACE EXPRESSION OF ENDOGENOUS HLA CLASS-I FREE HEAVY-CHAINS IN HCT HUMAN COLON-CARCINOMA CELLS, DEFICIENT IN BETA-2-MICROGLOBULIN". International Journal of Oncology 5.2 (1994): 181-188.
Chicago
SIMILE, M., CALORINI, L., RYAN, M., GATTONICELLI, S."INDUCTION BY HUMAN INTERFERON AND MURINE H-2L(D) GENE OF THE SURFACE EXPRESSION OF ENDOGENOUS HLA CLASS-I FREE HEAVY-CHAINS IN HCT HUMAN COLON-CARCINOMA CELLS, DEFICIENT IN BETA-2-MICROGLOBULIN". International Journal of Oncology 5, no. 2 (1994): 181-188. https://doi.org/10.3892/ijo.5.2.181