search button

PREPARATION AND PURIFICATION OF 3-FLUORO-TYROSINE LABELED TUMOR-NECROSIS-FACTOR-ALPHA AND PRELIMINARY F-19 N.M.R. INVESTIGATION

  • Authors:
    • JK SAGOO
    • LC CHAPLIN
    • NRA BEELEY
    • C IAONNOU
    • AM NESBITT
    • SJB TENDLER

  • View Affiliations

  • Published online on: October 1, 1994     https://doi.org/10.3892/ijo.5.4.937
  • Pages: 937-943
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A one-step protocol for the purification of recombinant human tumour necrosis factor-alpha (TNF alpha) has been developed based on the use of antibody affinity chromatography. The method allows for the preparation of large amounts of the protein (>15 mg). The overall recovery of the purified material from Esherichia coli lysate after buffer exchange into 0.8% mannitol is 48%, with no apparent loss of bioactivity. This method has been utilized for the preparation of 3-fluoro-tyrosine labelled human TNF alpha. Data indicate that the protein produced in minimal media is a heterotrimer consisting of two 17 kDa monomers and one proteolytically cleaved 14 kDa unit. Preliminary F-19 n.m.r. spectroscopy indicated that the 3-fluoro-tyrosine labelled protein is suitable for further study using this technique.

Related Articles

Journal Cover

October 1994
Volume 5 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
SAGOO J, CHAPLIN L, BEELEY N, IAONNOU C, NESBITT A and TENDLER S: PREPARATION AND PURIFICATION OF 3-FLUORO-TYROSINE LABELED TUMOR-NECROSIS-FACTOR-ALPHA AND PRELIMINARY F-19 N.M.R. INVESTIGATION. Int J Oncol 5: 937-943, 1994
APA
SAGOO, J., CHAPLIN, L., BEELEY, N., IAONNOU, C., NESBITT, A., & TENDLER, S. (1994). PREPARATION AND PURIFICATION OF 3-FLUORO-TYROSINE LABELED TUMOR-NECROSIS-FACTOR-ALPHA AND PRELIMINARY F-19 N.M.R. INVESTIGATION. International Journal of Oncology, 5, 937-943. https://doi.org/10.3892/ijo.5.4.937
MLA
SAGOO, J., CHAPLIN, L., BEELEY, N., IAONNOU, C., NESBITT, A., TENDLER, S."PREPARATION AND PURIFICATION OF 3-FLUORO-TYROSINE LABELED TUMOR-NECROSIS-FACTOR-ALPHA AND PRELIMINARY F-19 N.M.R. INVESTIGATION". International Journal of Oncology 5.4 (1994): 937-943.
Chicago
SAGOO, J., CHAPLIN, L., BEELEY, N., IAONNOU, C., NESBITT, A., TENDLER, S."PREPARATION AND PURIFICATION OF 3-FLUORO-TYROSINE LABELED TUMOR-NECROSIS-FACTOR-ALPHA AND PRELIMINARY F-19 N.M.R. INVESTIGATION". International Journal of Oncology 5, no. 4 (1994): 937-943. https://doi.org/10.3892/ijo.5.4.937