GENETIC ALTERATIONS IN P53 AND K-RAS IN LUNG-CANCER IN RELATION TO HISTOPATHOLOGY OF THE TUMOR AND SMOKING HISTORY OF THE PATIENT

  • Authors:
    • M RIDANPAA
    • A KARJALAINEN
    • S ANTTILA
    • H VAINIO
    • K HUSGAFVELPURSIAINEN
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  • Published online on: November 1, 1994     https://doi.org/10.3892/ijo.5.5.1109
  • Pages: 1109-1117
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Abstract

The inactivation of the p53 suppressor gene and the activation of the ras proto-oncogenes are frequent events in non-small cell lung cancer as well as in many other solid neoplasms in man. Somatic mutations in exons 5-8 of the p53 gene were detected in 59% (30/51) of the squamous cell carcinoma and in 38% (14/37) of the adenocarcinoma tumors using GC-clamped, non-radioactive denaturing gradient gel electrophoresis (DGGE). The mutations in the exons 5 and 8 represented larger proportion of the alterations in squamous cell carcinoma tumors (p=0.04; Fisher's exact test, two-tailed); in the adenocarcinoma tumors, mutations were most common in the exon 7 of p53. Most of the identified mutations (25/39; 64%) are predicted to cause an amino acid substitution. Mutations leading to the premature termination of translation were more frequent in adenocarcinoma (6/14) than in squamous cell carcinoma (3/30) tumors (p=0.02). In adenocarcinoma, also base substitutions in the K-ras gene were detected more often (18/37; 49%) than in squamous cell carcinoma (p<0.01). However, a mutation both in p53 and Kras was detected in only 4% of the lung tumors which does not support importance of co-operation between the genes in vivo. Mutations in p53 and K-ras did not correlate with tumor differentiation in either histological type. In squamous cell carcinoma, mutations in p53 showed relation to pack years smoked whereas in adenocarcinoma, mutations in the K-ras gene were associated with cigarette consumption. G to T transversion was the most common type of base substitution in both genes (31% in p53 and 53% in K-ras).

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November 1994
Volume 5 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
RIDANPAA, M., KARJALAINEN, A., ANTTILA, S., VAINIO, H., & HUSGAFVELPURSIAINEN, K. (1994). GENETIC ALTERATIONS IN P53 AND K-RAS IN LUNG-CANCER IN RELATION TO HISTOPATHOLOGY OF THE TUMOR AND SMOKING HISTORY OF THE PATIENT. International Journal of Oncology, 5, 1109-1117. https://doi.org/10.3892/ijo.5.5.1109
MLA
RIDANPAA, M., KARJALAINEN, A., ANTTILA, S., VAINIO, H., HUSGAFVELPURSIAINEN, K."GENETIC ALTERATIONS IN P53 AND K-RAS IN LUNG-CANCER IN RELATION TO HISTOPATHOLOGY OF THE TUMOR AND SMOKING HISTORY OF THE PATIENT". International Journal of Oncology 5.5 (1994): 1109-1117.
Chicago
RIDANPAA, M., KARJALAINEN, A., ANTTILA, S., VAINIO, H., HUSGAFVELPURSIAINEN, K."GENETIC ALTERATIONS IN P53 AND K-RAS IN LUNG-CANCER IN RELATION TO HISTOPATHOLOGY OF THE TUMOR AND SMOKING HISTORY OF THE PATIENT". International Journal of Oncology 5, no. 5 (1994): 1109-1117. https://doi.org/10.3892/ijo.5.5.1109