NON-POLARIZED EXPRESSION OF BASAL-CELL ADHESION MOLECULE B-CAM IN EPITHELIAL OVARIAN CANCERS

  • Authors:
    • P GARIN-CHESA
    • MP SANZ-MONCASI
    • IG CAMPBELL
    • WJ RETTIG
  • View Affiliations

  • Published online on: December 1, 1994     https://doi.org/10.3892/ijo.5.6.1261
  • Pages: 1261-1266
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Abstract

The basal cell adhesion molecule (B-CAM) is a M(r) 90,000 cell surface glycoprotein identified by two monoclonal antibodies (mAbs), F8 and G253, raised against human tumor cells. Cloning and sequence analysis of a B-CAM cDNA has revealed a characteristic immunoglobulin domain structure of the B-CAM polypeptide, most closely related to MUC18, a cell surface protein of invasive human melanomas. The pattern of B-CAM expression in cultured cells suggests that the molecule is associated with a substrate-adherent growth pattern in some lineages. Moreover, B-CAM expression is upregulated following malignant transformation in some cell types. In the present study, we have used immunohistochemical methods to examine S-CAM expression in normal and neoplastic tissues, including over 200 tumors of diverse histological type. B-CAM was detected in several normal tissues, including polarized expression in several epithelia and expression in vascular endothelium and smooth muscle. Among the tumors tested, B-CAM was found most uniformly and with a non-polarized pattern in epithelial cancers of the ovary (27 of 31 tumors B-CAM-positive), By contrast, only small subsets of epithelial cancers of other organs, including some neuroendocrine, breast, and lung carcinomas, showed uniform or polarized B-CAM expression. Most non-ovarian carcinomas, lymphomas, sarcomas, and neuroectodermal tumors tested were B-CAM-negative. Immunoprecipitation studies with ovarian carcinoma cell lines showed that B-CAM in these cells is a M, 90,000 glycoprotein, composed of M(r) 65,000-75,000 polypeptides with abundant, N-linked carbohydrate side chains. These findings identify B-CAM as a characteristic cell surface protein of epithelial ovarian cancers. The availability of B-CAM-specific mAbs and cDNAs may help identify the role of B-CAM in normal endothelial and epithelial cells and ovarian cancers.

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December 1994
Volume 5 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
GARIN-CHESA P, SANZ-MONCASI M, CAMPBELL I and RETTIG W: NON-POLARIZED EXPRESSION OF BASAL-CELL ADHESION MOLECULE B-CAM IN EPITHELIAL OVARIAN CANCERS. Int J Oncol 5: 1261-1266, 1994
APA
GARIN-CHESA, P., SANZ-MONCASI, M., CAMPBELL, I., & RETTIG, W. (1994). NON-POLARIZED EXPRESSION OF BASAL-CELL ADHESION MOLECULE B-CAM IN EPITHELIAL OVARIAN CANCERS. International Journal of Oncology, 5, 1261-1266. https://doi.org/10.3892/ijo.5.6.1261
MLA
GARIN-CHESA, P., SANZ-MONCASI, M., CAMPBELL, I., RETTIG, W."NON-POLARIZED EXPRESSION OF BASAL-CELL ADHESION MOLECULE B-CAM IN EPITHELIAL OVARIAN CANCERS". International Journal of Oncology 5.6 (1994): 1261-1266.
Chicago
GARIN-CHESA, P., SANZ-MONCASI, M., CAMPBELL, I., RETTIG, W."NON-POLARIZED EXPRESSION OF BASAL-CELL ADHESION MOLECULE B-CAM IN EPITHELIAL OVARIAN CANCERS". International Journal of Oncology 5, no. 6 (1994): 1261-1266. https://doi.org/10.3892/ijo.5.6.1261