Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer

  • Authors:
    • Lan Li
    • Daping Yang
    • Dejun Cui
    • Yu Li
    • Zhao Nie
    • Jinglin Wang
    • Li Liang
  • View Affiliations

  • Published online on: December 19, 2017     https://doi.org/10.3892/ijo.2017.4231
  • Pages:473-484
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Abstract

Gastric cancer, due to its high incidence rate, is the second leading cause of cancer-related mortality worldwide. Chemotherapy is an important component of the multimodal treatment for gastric cancer; however, a significant impediment to successful treatment is multidrug resistance (MDR) in patients with gastric cancer. In the present study, the protein profiles of the MDR cell line, SGC7901/DDP, and its parental cell line, SGC7901, were comparatively analyzed through an iTRAQ-based quantitative proteomics technique. The protein tetraspanin-8 (TSPAN8) was found to be highly expressed in the SGC7901/DDP cells. To examine the role of TSPAN8 in the MDR of SGC7901/DDP cells, we increased cell sensitivity to drugs by increasing apoptosis. Additionally, the silencing of TSPAN8 downregulated Wnt pathway activity, β-catenin expression and β-catenin transfer to the nucleus. TSPAN8 was found to bind to NOTCH2, facilitating its mediation of the Wnt/β-catenin pathway by regulating β-catenin expression. Overall, the suppression of TSPAN8 expression may prove to be a promising strategy which may aid in the development of novel gastric cancer therapeutic drugs.

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February 2018
Volume 52 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
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APA
Li, L., Yang, D., Cui, D., Li, Y., Nie, Z., Wang, J., & Liang, L. (2018). Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer. International Journal of Oncology, 52, 473-484. https://doi.org/10.3892/ijo.2017.4231
MLA
Li, L., Yang, D., Cui, D., Li, Y., Nie, Z., Wang, J., Liang, L."Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer". International Journal of Oncology 52.2 (2018): 473-484.
Chicago
Li, L., Yang, D., Cui, D., Li, Y., Nie, Z., Wang, J., Liang, L."Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer". International Journal of Oncology 52, no. 2 (2018): 473-484. https://doi.org/10.3892/ijo.2017.4231