Cyclooxygenase-2 expression is induced by celecoxib treatment in lung cancer cells and is transferred to neighbor cells via exosomes

  • Authors:
    • Jayoung Kim
    • Seung-Woo Hong
    • Seonghan Kim
    • Daejin Kim
    • Dae Young Hur
    • Dong-Hoon Jin
    • Bomi Kim
    • Yeong Seok Kim
  • View Affiliations

  • Published online on: December 13, 2017     https://doi.org/10.3892/ijo.2017.4227
  • Pages:613-620
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Abstract

Lung cancer is one of most common types of cancer worldwide. Lung cancer results in a death higher rate each year compared to colon, breast and prostate cancer combined. Celecoxib is a selective inhibitor of cyclooxygenase-2 (COX‑2), an enzyme of which the expression is induced by various stimuli, such as inflammation. In addition, celecoxib triggers COX-2 loading on exosomes. Exosomes are small vesicles composed of a lipid bilayer membrane and are found in most biological fluids, such as blood breast milk and urine. In this study, we focused on exosomes containing COX-2 proteins from lung cancer cells to determine their involvement in the interaction with neighbor cells following treatment with celecoxib. We found that celecoxib induced COX-2 expression in both the cytosol and exosomes in lung cancer cells. Exosomes from celecoxib-treated lung cancer cell culture supernatant were isolated and incubated with several types of cells. The THP-1, monocytic leukemia cell line effectively absorbed COX-2 by lung cancer cell-derived exosomes. Following incubation with exosomes, the COX-2 protein level was increased in the THP-1 cells; however, COX-2 mRNA expression was not affected. Moreover, prostaglandin E2 (PGE2) and vascular endothelial growth factor (VEGF) production by THP-1 cells was increased following incubation with exosomes from celecoxib-treated lung cancer cells. Conditioned medium from THP-1 following incubation with exosomes promoted formation in EA.hy926 cells. Taken together, our findings suggest that celecoxib induces COX-2 expression in lung cancer cells, and that highly expressed COX-2 in exosomes can be transferred to other cells.

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February 2018
Volume 52 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kim, J., Hong, S., Kim, S., Kim, D., Hur, D.Y., Jin, D. ... Kim, Y.S. (2018). Cyclooxygenase-2 expression is induced by celecoxib treatment in lung cancer cells and is transferred to neighbor cells via exosomes. International Journal of Oncology, 52, 613-620. https://doi.org/10.3892/ijo.2017.4227
MLA
Kim, J., Hong, S., Kim, S., Kim, D., Hur, D. Y., Jin, D., Kim, B., Kim, Y. S."Cyclooxygenase-2 expression is induced by celecoxib treatment in lung cancer cells and is transferred to neighbor cells via exosomes". International Journal of Oncology 52.2 (2018): 613-620.
Chicago
Kim, J., Hong, S., Kim, S., Kim, D., Hur, D. Y., Jin, D., Kim, B., Kim, Y. S."Cyclooxygenase-2 expression is induced by celecoxib treatment in lung cancer cells and is transferred to neighbor cells via exosomes". International Journal of Oncology 52, no. 2 (2018): 613-620. https://doi.org/10.3892/ijo.2017.4227