High expression of Copine 1 promotes cell growth and metastasis in human lung adenocarcinoma

Liu,Shunlin; Tang,Haicheng; Zhu,Jianjie; Ding,Heguo; Zeng,Yuanyuan; Du,Wenwen; Ding,Zongli; Song,Pengtao; Zhang,Yang; Liu,Zeyi; Huang,Jian-An

doi:10.3892/ijo.2018.4558

High expression of Copine 1 promotes cell growth and metastasis in human lung adenocarcinoma

Authors:
- Shunlin Liu
- Haicheng Tang
- Jianjie Zhu
- Heguo Ding
- Yuanyuan Zeng
- Wenwen Du
- Zongli Ding
- Pengtao Song
- Yang Zhang
- Zeyi Liu
- Jian-An Huang
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Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Respiratory Medicine, Huzhou 3rd Hospital, Huzhou, Zhejiang 313000, P.R. China, Department of Pathology, Huzhou 3rd Hospital, Huzhou, Zhejiang 313000, P.R. China
Published online on: September 12, 2018 https://doi.org/10.3892/ijo.2018.4558
Pages: 2369-2378
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Despite advances in diagnosis and treatment, the survival of non-small cell lung cancer (NSCLC) patients is poor. Further understanding of the disease mechanism and treatment strategies is required. Copines are a family of calcium-dependent phospholipid-binding proteins that are evolutionally conserved in various eukaryotic organisms and protists. Copine 1, encoded by CPNE1, is a soluble membrane-binding protein, which includes two tandem C2 domains at the N-terminus and an A domain at the C‑terminus. A previous study reported that Copine 1 binds with various intracellular proteins via its A domain and C omain. However, the role of CPNE1 in lung cancer remains unclear. In the presented study, CPNE1 expression level was demonstrated to be positively associated with the stage (P=0.002) and significantly associated with lymph node status (P=0.011) and distant metastasis (P=0.042). Furthermore, the function of CPNE1 in regulation of cell growth, migration and invasion was investigated, and it was demonstrated that knockdown of CPNE1 inhibits the cell cycle in NSCLC cells. Collectively, these data suggest that CPNE1 is an oncogene in NSCLC and serves an important role in tumorigenesis of NSCLC progression.

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