Open Access

COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer

  • Authors:
    • Kwabena Gyabaah Owusu‑Ansah
    • Guangyuan Song
    • Ronggao Chen
    • Muhammad Ibrahim Alhadi Edoo
    • Jun Li
    • Bingjie Chen
    • Jian Wu
    • Lin Zhou
    • Haiyang Xie
    • Donghai Jiang
    • Shusen Zheng
  • View Affiliations

  • Published online on: June 14, 2019     https://doi.org/10.3892/ijo.2019.4825
  • Pages: 391-404
  • Copyright: © Owusu‑Ansah et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pancreatic cancer is one of the most aggressive cancers worldwide with a high mortality rate. Prognosis remains poor even in this era of advanced medicine mainly due to early metastasis and invasion. The present study aimed to explore and validate predictors of distant metastasis and prognosis in pancreatic cancer. In our preliminary experiment, we established a novel metastatic pancreatic cancer cell line BxPC‑M8 from parent BxPC‑3 cells. Via whole genome sequencing, RT‑qPCR, western blotting, migration and invasion assays, we initially found that BxPC‑M8 shared similar biological characteristics to BxPC‑3, but only differed in enhanced metastatic and invasive capabilities with a significant increase in collagen type VI α1 chain (COL6A1) expression. Knockdown of COL6A1 via small interfering RNA led to a significant decrease in migration and invasion of BxPC‑M8 cells, suggesting suppressed epithelial‑mesenchymal transition. Furthermore, a significant increase in COL6A1 expression was observed in cancerous tissue compared with paracancerous tissue (40.7 vs 3.7, P=0.001). Additionally, its expression was observed to be significantly associated with distant metastasis and vascular invasion at the time of surgery. Multivariate analysis revealed that COL6A1 expression (hazard ratio 1.90, 95% confidence interval 1.04‑3.47, P=0.037) is an independent predictor of overall survival (OS). The median OS observed for COL6A1+ and COL6A1‑ patients was found to be 8±4 and 14±7 months (P=0.021), respectively. Of note, we identified that COL6A1 expression in tissue samples was associated with significantly reduced OS (P=0.001), demonstrating that COL6A1 may serve an important role in the metastatic process and could be considered as a predictor of poor outcomes in patients with pancreatic cancer. In addition, our findings suggest that COL6A1 could be an indicator of distant metastasis and a valid prognostic predictor in such patients; however, further investigation is required.
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August-2019
Volume 55 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Owusu‑Ansah KG, Song G, Chen R, Edoo MI, Li J, Chen B, Wu J, Zhou L, Xie H, Jiang D, Jiang D, et al: COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer . Int J Oncol 55: 391-404, 2019
APA
Owusu‑Ansah, K.G., Song, G., Chen, R., Edoo, M.I., Li, J., Chen, B. ... Zheng, S. (2019). COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer . International Journal of Oncology, 55, 391-404. https://doi.org/10.3892/ijo.2019.4825
MLA
Owusu‑Ansah, K. G., Song, G., Chen, R., Edoo, M. I., Li, J., Chen, B., Wu, J., Zhou, L., Xie, H., Jiang, D., Zheng, S."COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer ". International Journal of Oncology 55.2 (2019): 391-404.
Chicago
Owusu‑Ansah, K. G., Song, G., Chen, R., Edoo, M. I., Li, J., Chen, B., Wu, J., Zhou, L., Xie, H., Jiang, D., Zheng, S."COL6A1 promotes metastasis and predicts poor prognosis in patients with pancreatic cancer ". International Journal of Oncology 55, no. 2 (2019): 391-404. https://doi.org/10.3892/ijo.2019.4825