RADIOSENSITIZATION BY CISPLATIN TREATMENT IN CISPLATIN-RESISTANT AND SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES

  • Authors:
    • GP RAAPHORST
    • G WANG
    • CE NG
  • View Affiliations

  • Published online on: August 1, 1995     https://doi.org/10.3892/ijo.7.2.325
  • Pages: 325-330
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The responses of cisplatin resistant (A2780(cp)) and cisplatin sensitive (A2780) ovarian carcinoma cell lines to radiation, cisplatin and cisplatin plus radiation have been studied. The cisplatin resistant cell line showed cross resistance to radiation. When cells were exposed to 2 mu g/ml of cisplatin treatment for one hour radiosensitization was achieved. The degree of radiosensitization was treatment sequence dependent. Irradiation followed by cisplatin treatment resulted in synergistic interaction with dose modifying factors (DMFs) as high as 1.5 in the resistant line and 1.1 in the sensitive line. The reverse sequence resulted in antagonistic interaction with DMFs of 0.8 to 0.9 at the 10% survival level. At lower survival levels the interaction remained antagonistic for the resistant cell line. Increasing the cisplatin concentration to 4 mu g/ml did not increase the degree of cisplatin radiosensitization. Simultaneous treatment of irradiation during the middle of a one or two hour cisplatin treatment did not increase the degree of radiosensitization. The cisplatin resistant cell line had a much greater cisplatin radiosensitization effect than the sensitive parental cell line with maximum DMFs for the resistant cell line ranging from 0.9-1.5 and for the sensitive cell line from 0.8-1.1. These results indicate that cisplatin may be an effective radiosensitizer especially in cisplatin resistant cell lines. This effect may be related to the inhibition of repair of radiation damage which may be elevated as a mechanism of resistance in the resistant cell line.

Related Articles

Journal Cover

August 1995
Volume 7 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
RAAPHORST G, WANG G and NG C: RADIOSENSITIZATION BY CISPLATIN TREATMENT IN CISPLATIN-RESISTANT AND SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES. Int J Oncol 7: 325-330, 1995
APA
RAAPHORST, G., WANG, G., & NG, C. (1995). RADIOSENSITIZATION BY CISPLATIN TREATMENT IN CISPLATIN-RESISTANT AND SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES. International Journal of Oncology, 7, 325-330. https://doi.org/10.3892/ijo.7.2.325
MLA
RAAPHORST, G., WANG, G., NG, C."RADIOSENSITIZATION BY CISPLATIN TREATMENT IN CISPLATIN-RESISTANT AND SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES". International Journal of Oncology 7.2 (1995): 325-330.
Chicago
RAAPHORST, G., WANG, G., NG, C."RADIOSENSITIZATION BY CISPLATIN TREATMENT IN CISPLATIN-RESISTANT AND SENSITIVE HUMAN OVARIAN-CARCINOMA CELL-LINES". International Journal of Oncology 7, no. 2 (1995): 325-330. https://doi.org/10.3892/ijo.7.2.325