COMPARISON OF ADHERENT LYMPHOKINE-ACTIVATED KILLER (A-LAK) CELLS GENERATED BY IL-2 AND IL-7 - CELLULAR MODIFICATIONS INDUCED BY IL-7

  • Authors:
    • P MOLLER
    • M BOHM
    • S KRUGERKRASAGAKES
    • C KOHLMUS
    • M ARTUC
    • BM CZARNETZKI
    • D SCHADENDORF
  • View Affiliations

  • Published online on: August 1, 1995     https://doi.org/10.3892/ijo.7.2.383
  • Pages: 383-389
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Abstract

At present, the clinical application of plastic-adherent-lymphokine-activated killer (A-LAK) cells shows limited success in the immunotherapy of patients with advanced cancer because of a low responder rate, severe side effects and failures in yielding sufficient numbers of cells for adoptive transfers. Since interleukin-7 (IL-7) is able to induce LAK activity independently of IL-2, we investigated the ability of IL-7 to improve the yield and the properties of A-LAK cells. A-LAK cells from 7 healthy donors generated in the presence of IL-2, IL-7 or combinations of IL-2 plus IL-7 (each 1000 U/ml) were compared with regard to plastic adherence, expansion rate, immunophenotype, cytokine secretion and cytotoxicity against malignant melanoma cells and non-malignant target cells. Our results demonstrate that A-LAK cells generated by a simultaneous stimulation of IL-2 plus IL-7 displayed a significantly higher expansion rate (10.7-fold vs. 9.0-fold), but showed no difference in the cytolytic activity compared to A-LAK cells generated by IL-2 alone. A-LAK cells generated by IL-7 alone demonstrated a low expansion rate (1.1-fold vs. 8.8-fold), and decreased in other properties like plastic adherence, CD56(+)/CD3(+) cell-ratio and cytolytic activity compared to A-LAK cells generated by IL-2 alone. A-LAK cells generated by IL-7 or a sequential stimulation of IL-2 and IL-7, on the other hand, exhibited a more selective cytotoxicity for malignant melanoma cells compared to the non-malignant keratinocyte target cell line (HaCaT) and normal fibroblasts. A sequential replacement of LL-2 by IL-7 might help to reduce the severe side effects of IL-2. In vivo experiments are necessary to evaluate the potential value of IL-7 in adoptive immunotherapy.

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August 1995
Volume 7 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
MOLLER P, BOHM M, KRUGERKRASAGAKES S, KOHLMUS C, ARTUC M, CZARNETZKI B and SCHADENDORF D: COMPARISON OF ADHERENT LYMPHOKINE-ACTIVATED KILLER (A-LAK) CELLS GENERATED BY IL-2 AND IL-7 - CELLULAR MODIFICATIONS INDUCED BY IL-7. Int J Oncol 7: 383-389, 1995
APA
MOLLER, P., BOHM, M., KRUGERKRASAGAKES, S., KOHLMUS, C., ARTUC, M., CZARNETZKI, B., & SCHADENDORF, D. (1995). COMPARISON OF ADHERENT LYMPHOKINE-ACTIVATED KILLER (A-LAK) CELLS GENERATED BY IL-2 AND IL-7 - CELLULAR MODIFICATIONS INDUCED BY IL-7. International Journal of Oncology, 7, 383-389. https://doi.org/10.3892/ijo.7.2.383
MLA
MOLLER, P., BOHM, M., KRUGERKRASAGAKES, S., KOHLMUS, C., ARTUC, M., CZARNETZKI, B., SCHADENDORF, D."COMPARISON OF ADHERENT LYMPHOKINE-ACTIVATED KILLER (A-LAK) CELLS GENERATED BY IL-2 AND IL-7 - CELLULAR MODIFICATIONS INDUCED BY IL-7". International Journal of Oncology 7.2 (1995): 383-389.
Chicago
MOLLER, P., BOHM, M., KRUGERKRASAGAKES, S., KOHLMUS, C., ARTUC, M., CZARNETZKI, B., SCHADENDORF, D."COMPARISON OF ADHERENT LYMPHOKINE-ACTIVATED KILLER (A-LAK) CELLS GENERATED BY IL-2 AND IL-7 - CELLULAR MODIFICATIONS INDUCED BY IL-7". International Journal of Oncology 7, no. 2 (1995): 383-389. https://doi.org/10.3892/ijo.7.2.383