INHIBITION OF PKC AND PKA BY CHEMOPREVENTIVE ORGANOSELENIUM COMPOUNDS

  • Authors:
    • PG FOILES
    • H FUJIKI
    • M SUGANUMA
    • S OKABE
    • J YATSUNAMI
    • LM MIGLIETTA
    • P UPADHYAYA
    • K ELBAYOUMY
    • Z RONAI
  • View Affiliations

  • Published online on: September 1, 1995     https://doi.org/10.3892/ijo.7.3.685
  • Pages: 685-690
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Abstract

Numerous efficacy studies in rodents revealed that 1,4-phenylenebis(methylene)-selenocyanat (p-XSC) is a more effective chemopreventive organoselenium compound and less toxic than benzyl selenocyanate (BSC) or the inorganic compound Na2SeO3. To explore mechanisms which mediate chemopreventive activities of p-XSC we have tested its effect on protein kinase A and C using in vitro and cell culture systems. While p-XSC did completely inhibit PKC and PKA activity, BSC was less active and Na2SeO3 had no effect. Comparative EC,, revealed values of 0.1, 1 and > 10 mu M for p-XSC, BSC and Na2SeO3, respectively. p-XSC was also capable of inhibiting protein phosphorylation in cultures of primary human fibroblasts and altered morphology of rat fibroblast (R6) cells. When combined, sub-optimal doses of p-XSC and staurosporine yielded an additive effect on cell morphology. The ability of p-XSC and BSC to inhibit protein kinase A and C activities may in part account for the mechanism(s) by which these agents mediate their chemopreventive effects.

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September 1995
Volume 7 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
FOILES P, FUJIKI H, SUGANUMA M, OKABE S, YATSUNAMI J, MIGLIETTA L, UPADHYAYA P, ELBAYOUMY K and RONAI Z: INHIBITION OF PKC AND PKA BY CHEMOPREVENTIVE ORGANOSELENIUM COMPOUNDS. Int J Oncol 7: 685-690, 1995.
APA
FOILES, P., FUJIKI, H., SUGANUMA, M., OKABE, S., YATSUNAMI, J., MIGLIETTA, L. ... RONAI, Z. (1995). INHIBITION OF PKC AND PKA BY CHEMOPREVENTIVE ORGANOSELENIUM COMPOUNDS. International Journal of Oncology, 7, 685-690. https://doi.org/10.3892/ijo.7.3.685
MLA
FOILES, P., FUJIKI, H., SUGANUMA, M., OKABE, S., YATSUNAMI, J., MIGLIETTA, L., UPADHYAYA, P., ELBAYOUMY, K., RONAI, Z."INHIBITION OF PKC AND PKA BY CHEMOPREVENTIVE ORGANOSELENIUM COMPOUNDS". International Journal of Oncology 7.3 (1995): 685-690.
Chicago
FOILES, P., FUJIKI, H., SUGANUMA, M., OKABE, S., YATSUNAMI, J., MIGLIETTA, L., UPADHYAYA, P., ELBAYOUMY, K., RONAI, Z."INHIBITION OF PKC AND PKA BY CHEMOPREVENTIVE ORGANOSELENIUM COMPOUNDS". International Journal of Oncology 7, no. 3 (1995): 685-690. https://doi.org/10.3892/ijo.7.3.685