Heparin-binding EGF-like growth factor expression and biological action in human pancreatic cancer cells

  • Authors:
    • M Yokoyama
    • H Funatomi
    • C Hope
    • D Damm
    • H Friess
    • M Buchler
    • J Abraham
    • M Korc
  • View Affiliations

  • Published online on: February 1, 1996     https://doi.org/10.3892/ijo.8.2.289
  • Pages: 289-295
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The epidermal growth factor (EGF) receptor is activated by EGF and other EGF-like growth factors, including heparin-binding epidermal growth factor-like growth factor (HB-EGF). We characterized the biological actions of HB-EGF in PANC-1 and COLO-357 human pancreatic cancer cell lines, and determined whether the presence of HB-EGF in human pancreatic carcinomas correlates with patient survival. HB-EGF enhanced the growth of both cell lines in a dose-dependent manner, with a potency that was generally similar to that of EGF and transforming growth factor-alpha (TGF-alpha). HB-EGF also readily induced tyrosine phosphorylation of the EGF receptor in these cells. Immunohistochemical analysis of 47 pancreatic cancer tissues revealed the presence of HB-EGF immunoreactivity in the cancer cells in 50% of the tumors. However, the presence of HB-EGF was not associated with a statistically significant decrease in the post-operative survival period. Furthermore, coexpression of HB-EGF and the EGF receptor was not associated with shorter patient survival. These findings suggest that HB-EGF activates the EGF receptor in human pancreatic cancer cells, but that it is not involved in enhancing the biological aggressiveness of this malignancy in vivo.

Related Articles

Journal Cover

February 1996
Volume 8 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yokoyama M, Funatomi H, Hope C, Damm D, Friess H, Buchler M, Abraham J and Korc M: Heparin-binding EGF-like growth factor expression and biological action in human pancreatic cancer cells. Int J Oncol 8: 289-295, 1996.
APA
Yokoyama, M., Funatomi, H., Hope, C., Damm, D., Friess, H., Buchler, M. ... Korc, M. (1996). Heparin-binding EGF-like growth factor expression and biological action in human pancreatic cancer cells. International Journal of Oncology, 8, 289-295. https://doi.org/10.3892/ijo.8.2.289
MLA
Yokoyama, M., Funatomi, H., Hope, C., Damm, D., Friess, H., Buchler, M., Abraham, J., Korc, M."Heparin-binding EGF-like growth factor expression and biological action in human pancreatic cancer cells". International Journal of Oncology 8.2 (1996): 289-295.
Chicago
Yokoyama, M., Funatomi, H., Hope, C., Damm, D., Friess, H., Buchler, M., Abraham, J., Korc, M."Heparin-binding EGF-like growth factor expression and biological action in human pancreatic cancer cells". International Journal of Oncology 8, no. 2 (1996): 289-295. https://doi.org/10.3892/ijo.8.2.289