Cell density-dependent modulation of basic fibroblast growth factor expression by human interferon-beta

  • Authors:
    • R Singh
    • C Bucana
    • N Llansa
    • R Sanchez
    • I Fidler
  • View Affiliations

  • Published online on: April 1, 1996     https://doi.org/10.3892/ijo.8.4.649
  • Pages: 649-656
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Abstract

The production of basic fibroblast growth factor (bFGF) by human renal cell carcinoma (HRCC) is associated with induction of angiogenesis. Incubation of HRCC cells with human interferon alpha (IFN-alpha) or interferon beta (IFN-beta) downregulates the expression of bFGF and, hence, angiogenesis. The purpose of this study was to analyze the downregulation of the expression of bFGF in HRCC cells by IFN-alpha and IFN-beta. Human HRCC SN12PM6 cells cultured under sparse conditions expressed 3-7-fold higher levels of steady-state bFGF-specific mRNA transcripts and cellular bFGF protein than did confluent cultures. IFN-alpha or IFN-beta inhibited the steady-state expression of bFGF mRNA transcripts and cellular bFGF protein in a concentration-dependent manner in sparse but not confluent cultures. Moreover, IFN-beta downregulated the transcription rate of bFGF genes and inhibited the de novo synthesis of bFGF protein only in sparse cultures. The results demonstrate that the inhibitory effects of IFN- and -beta on bFGF expression are cell-density dependent.

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April 1996
Volume 8 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Singh, R., Bucana, C., Llansa, N., Sanchez, R., & Fidler, I. (1996). Cell density-dependent modulation of basic fibroblast growth factor expression by human interferon-beta. International Journal of Oncology, 8, 649-656. https://doi.org/10.3892/ijo.8.4.649
MLA
Singh, R., Bucana, C., Llansa, N., Sanchez, R., Fidler, I."Cell density-dependent modulation of basic fibroblast growth factor expression by human interferon-beta". International Journal of Oncology 8.4 (1996): 649-656.
Chicago
Singh, R., Bucana, C., Llansa, N., Sanchez, R., Fidler, I."Cell density-dependent modulation of basic fibroblast growth factor expression by human interferon-beta". International Journal of Oncology 8, no. 4 (1996): 649-656. https://doi.org/10.3892/ijo.8.4.649