Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells

  • Authors:
    • E Venturelli
    • D Coradini
    • D Gornati
    • G Secreto
  • View Affiliations

  • Published online on: April 1, 1996     https://doi.org/10.3892/ijo.8.4.687
  • Pages: 687-692
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The purpose of our study was to evaluate the effect of testosterone and its metabolic pathway in MCF-7 cells in culture. Testosterone exhibited a dose-dependent (from 0.1 to 10 nM) and time-dependent (from 3 to 9 days) growth stimulation. The metabolic pathway was investigated following treatment with two testosterone concentrations: one stimulating (10 nM) and one not affecting (0.1 nM) cell growth. Celite column chromatography was used to separate H-3-testosterone metabolites, whose identity was confirmed by gas chromatography-mass spectrometry analysis. The main findings of the metabolic study were: i) recovery of a large amount of untransformed testosterone; ii) a high conversion of testosterone to conjugated, biologically inactive metabolites; iii) the highest level of Sa-diol among the metabolites of testosterone; iv) a conversion (2%) of testosterone into oestradiol, which resulted in a growth stimulatory concentration when testosterone was used at 10 nM. We conclude that in our experimental conditions androgens and oestrogens can concur to stimulate MCF-7 cell growth through androgen receptor-mediated and oestrogen receptor-mediated mechanisms.

Related Articles

Journal Cover

April 1996
Volume 8 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Venturelli, E., Coradini, D., Gornati, D., & Secreto, G. (1996). Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells. International Journal of Oncology, 8, 687-692. https://doi.org/10.3892/ijo.8.4.687
MLA
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8.4 (1996): 687-692.
Chicago
Venturelli, E., Coradini, D., Gornati, D., Secreto, G."Growth stimulatory effect and metabolism of testosterone in MCF-7 human breast cancer cells". International Journal of Oncology 8, no. 4 (1996): 687-692. https://doi.org/10.3892/ijo.8.4.687