Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells

  • Authors:
    • S Joshi
    • A Wu
    • D Verbik
    • S Algarra
    • M Bishop
    • S Pirruccello
    • P Iversen
    • J Jackson
    • M Kessinger
    • J Sharp
  • View Affiliations

  • Published online on: April 1, 1996     https://doi.org/10.3892/ijo.8.4.815
  • Pages: 815-820
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Abstract

A 24-mer (antisense) phosphorothioate oligonucleotide (ODN) corresponding to the codons 2-9 of the c-myb gene was evaluated for its effects on the growth of a human Burkitt lymphoma cell line (Raji) in vitro. Raji cells incubated with different concentrations of c-myb antisense ODN (5-15 mu g/ml) for 24-72 h showed a significant dose-dependent decrease in growth. The same concentrations of control (sense) or scrambled c-myb phosphorothioate ODNs did not inhibit Raji cell growth. The c-myb antisense ODN, but not the control ODNs, significantly decreased c-myb mRNA levels in treated cells as determined by RT-PCR. Additionally, the c-myb antisense ODN induced apoptosis of Raji cells as demonstrated by i) flow cytometry to enumerate the A(o) (apoptotic cell population) population of propidium iodide stained cells; ii) electron microscopy to evaluate the cell morphology; and iii) DNA fragmentation pattern. Thus, an antisense c-myb ODN causes significant growth inhibition of Burkitt lymphoma cells, and one mechanism of growth inhibition is the induction of apoptosis of the lymphoma cells. In addition, antisense c-myb ODN did not reduce CFU-GM or BFU-e colony-forming ability of normal hematopoietic stem/progenitor cells. Because the inhibition is sequence-specific and Burkitt lymphoma cell selective, evaluation of the therapeutic effects of c-myb antisense ODN against Burkitt lymphoma is warranted.

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April 1996
Volume 8 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Joshi, S., Wu, A., Verbik, D., Algarra, S., Bishop, M., Pirruccello, S. ... Sharp, J. (1996). Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells. International Journal of Oncology, 8, 815-820. https://doi.org/10.3892/ijo.8.4.815
MLA
Joshi, S., Wu, A., Verbik, D., Algarra, S., Bishop, M., Pirruccello, S., Iversen, P., Jackson, J., Kessinger, M., Sharp, J."Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells". International Journal of Oncology 8.4 (1996): 815-820.
Chicago
Joshi, S., Wu, A., Verbik, D., Algarra, S., Bishop, M., Pirruccello, S., Iversen, P., Jackson, J., Kessinger, M., Sharp, J."Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells". International Journal of Oncology 8, no. 4 (1996): 815-820. https://doi.org/10.3892/ijo.8.4.815