Early responses of SiHa human squamous carcinoma cells to hypoxic signals

  • Authors:
    • K Laderoute
    • J Calaoagan
    • H Mendonca
    • W Ausserer
    • E Chen
    • A Giaccia
    • R Sutherland
  • View Affiliations

  • Published online on: May 1, 1996     https://doi.org/10.3892/ijo.8.5.875
  • Pages: 875-882
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We compared the responses of c-Jun/AP-1 transcriptional complexes with those of NF-kappa B, an established hypoxia-inducible transcriptional complex, in hypoxic SiHa human squamous carcinoma cells. We observed that NF-kappa B was activated rapidly, while AP-1 activation was detectable only after prolonged hypoxia. However, in parallel with NF-kappa B activation, hypoxia induced a protein kinase activity that could phosphorylate the transactivation domain of the ATF-2 transcription factor in vitro. Taken together, these experiments indicate that NF-kappa B can rapidly transduce hypoxic signals through increased DNA-binding and transactivation activities, whereas specific AP-1 (ATF-2/c-Jun) complexes may be activated under the same hypoxic conditions by a stress-reponsive MAPK pathway.

Related Articles

Journal Cover

May 1996
Volume 8 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Laderoute K, Calaoagan J, Mendonca H, Ausserer W, Chen E, Giaccia A and Sutherland R: Early responses of SiHa human squamous carcinoma cells to hypoxic signals. Int J Oncol 8: 875-882, 1996
APA
Laderoute, K., Calaoagan, J., Mendonca, H., Ausserer, W., Chen, E., Giaccia, A., & Sutherland, R. (1996). Early responses of SiHa human squamous carcinoma cells to hypoxic signals. International Journal of Oncology, 8, 875-882. https://doi.org/10.3892/ijo.8.5.875
MLA
Laderoute, K., Calaoagan, J., Mendonca, H., Ausserer, W., Chen, E., Giaccia, A., Sutherland, R."Early responses of SiHa human squamous carcinoma cells to hypoxic signals". International Journal of Oncology 8.5 (1996): 875-882.
Chicago
Laderoute, K., Calaoagan, J., Mendonca, H., Ausserer, W., Chen, E., Giaccia, A., Sutherland, R."Early responses of SiHa human squamous carcinoma cells to hypoxic signals". International Journal of Oncology 8, no. 5 (1996): 875-882. https://doi.org/10.3892/ijo.8.5.875