Differential expression of P-glycoprotein in cord blood and peripheral blood lymphocyte subpopulations

  • Authors:
    • S Aggarwal
    • A Gupta
    • T Tsuruo
    • S Gupta
  • View Affiliations

  • Published online on: June 1, 1996     https://doi.org/10.3892/ijo.8.6.1063
  • Pages: 1063-1068
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

P-glycoprotein (P-gP) is a 170 kDa glycoprotein encoded by MDR1 that appears to be involved in the secretion of a large number of molecules including hormones, cyclosporin A (CSA) and cytokines. Cord blood lymphocytes are functionally immature and are sensitive to CSA. Therefore, we compared the surface expression and function of P-gP in cord blood and peripheral blood CD4(+) and CD8(+) T cells and their naive (CD45RA(+)) and memory (CD45RO(+)) subsets. We observed that only 1% of cord blood CD4(+) and 2% CD8(+) cells expressed P-gP as compared to 7% and 10% respectively of peripheral blood from young controls. In addition, MDR1 mRNA levels were also decreased in cord blood mononuclear cells as compared to peripheral blood from young controls. The efflux function of P-gP was measured by intracellular accumulation of rhodamine 123 (Rh123, a substrate for P-gP) in the presence or absence of CSA. A higher accumulation of Rh123 was observed in cord blood CD4(+) and CD8(+) and their naive and memory cell subtype as compared to those in peripheral blood. Furthermore, cord blood CD4(+) and CD8(+) T cell subsets exhibited greater sensitivity towards CSA (% increase: 119 and 188 respectively) as compared to peripheral blood (44 and 89 respectively). A physiological role of decreased P-gP expression and function in cord blood remains to be determined.

Related Articles

Journal Cover

June 1996
Volume 8 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Aggarwal S, Gupta A, Tsuruo T and Gupta S: Differential expression of P-glycoprotein in cord blood and peripheral blood lymphocyte subpopulations. Int J Oncol 8: 1063-1068, 1996.
APA
Aggarwal, S., Gupta, A., Tsuruo, T., & Gupta, S. (1996). Differential expression of P-glycoprotein in cord blood and peripheral blood lymphocyte subpopulations. International Journal of Oncology, 8, 1063-1068. https://doi.org/10.3892/ijo.8.6.1063
MLA
Aggarwal, S., Gupta, A., Tsuruo, T., Gupta, S."Differential expression of P-glycoprotein in cord blood and peripheral blood lymphocyte subpopulations". International Journal of Oncology 8.6 (1996): 1063-1068.
Chicago
Aggarwal, S., Gupta, A., Tsuruo, T., Gupta, S."Differential expression of P-glycoprotein in cord blood and peripheral blood lymphocyte subpopulations". International Journal of Oncology 8, no. 6 (1996): 1063-1068. https://doi.org/10.3892/ijo.8.6.1063