Human and rodent expression pattern of a fusion gene isolated from an MCF7 cDNA library

  • Authors:
    • J Reichert
    • N Blin
  • View Affiliations

  • Published online on: July 1, 1996     https://doi.org/10.3892/ijo.9.1.29
  • Pages: 29-32
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Abstract

A cDNA library established from the human mammary carcinoma cell line MCF7 was used to screen for transcripts related to or representing the gene coding for the estrogen receptor-related protein ERD5. Among the two populations of cDNAs isolated, one group (3 clones) represented heat shock protein transcripts (HSP27), the other (2 clones) appeared identical in their insert size and restriction enzyme banding pattern. Detailed analysis of one of them revealed its nature as a fusion sequence composed of two genes. At the 5'-end it consists of the last 157 bp of the HSP28 gene. At its 3'-part, it is identical to the 3'-portion of thr nuclear factor IV gene. The genuine expression of this fusion gene was demonstrated by Northern blots and RT-PCR. It appears to be ubiquitously expressed in a variety of normal tissues and malignant cells in man, mouse and rat.

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July 1996
Volume 9 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Reichert J and Reichert J: Human and rodent expression pattern of a fusion gene isolated from an MCF7 cDNA library. Int J Oncol 9: 29-32, 1996
APA
Reichert, J., & Reichert, J. (1996). Human and rodent expression pattern of a fusion gene isolated from an MCF7 cDNA library. International Journal of Oncology, 9, 29-32. https://doi.org/10.3892/ijo.9.1.29
MLA
Reichert, J., Blin, N."Human and rodent expression pattern of a fusion gene isolated from an MCF7 cDNA library". International Journal of Oncology 9.1 (1996): 29-32.
Chicago
Reichert, J., Blin, N."Human and rodent expression pattern of a fusion gene isolated from an MCF7 cDNA library". International Journal of Oncology 9, no. 1 (1996): 29-32. https://doi.org/10.3892/ijo.9.1.29