Dysregulation of transforming growth factor-beta 1 expression in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis

  • Authors:
    • Q Wang
    • C Sabourin
    • L Kresty
    • G Stoner
  • View Affiliations

  • Published online on: September 1, 1996     https://doi.org/10.3892/ijo.9.3.473
  • Pages: 473-479
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Abstract

Alterations in the expression of transforming growth factor-beta 1 (TGF-beta 1) have been implicated for malignancies of both human and experimental animal models. To further investigate the role of TGF-beta 1 in chemically-induced esophageal tumorigenesis, the expression of TGF-beta 1 was analyzed by reverse transcription-polymerase chain reaction (T-PCR) and immunohistochemical studies in N-nitrosomethylbenzylamine (NMBA)-induced rat esophageal tumorigenesis. We observed no significant difference in TGF-beta 1 mRNA levels between 25-week papillomas and normal rat esophageal epithelium. However, TGF-beta 1 mRNA levels were increased 2.9-fold (p<0.05) in 45-week papillomas induced by NMBA, when compared to normal rat esophageal epithelium. Immunohistochemical studies revealed a decreased intracellular TGF-beta 1 immunoreactivity in the papillomas when compared to the normal rat esophageal epithelium, indicating the existence of a discordance between TGF-beta 1 mRNA and protein expression. Furthermore, by immunohistochemical staining of proliferating cell nuclear antigen (PCNA), a predominantely mutually exclusive staining pattern was observed for TGF-beta 1 and PCNA. These findings suggest that overexpression of TGF-beta 1 mRNA occurs late in NMBA-induced rat esophageal tumorigenesis, and the discordant mRNA and protein expression may result in tumor evasion from TGF-beta 1 controlled negative growth regulation, thus promoting tumor progression in this model.

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September 1996
Volume 9 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang Q, Sabourin C, Kresty L and Stoner G: Dysregulation of transforming growth factor-beta 1 expression in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis. Int J Oncol 9: 473-479, 1996.
APA
Wang, Q., Sabourin, C., Kresty, L., & Stoner, G. (1996). Dysregulation of transforming growth factor-beta 1 expression in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis. International Journal of Oncology, 9, 473-479. https://doi.org/10.3892/ijo.9.3.473
MLA
Wang, Q., Sabourin, C., Kresty, L., Stoner, G."Dysregulation of transforming growth factor-beta 1 expression in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis". International Journal of Oncology 9.3 (1996): 473-479.
Chicago
Wang, Q., Sabourin, C., Kresty, L., Stoner, G."Dysregulation of transforming growth factor-beta 1 expression in N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis". International Journal of Oncology 9, no. 3 (1996): 473-479. https://doi.org/10.3892/ijo.9.3.473