Quantification of microvessel density in human tumours

Abdalla,S; Haboubi,N; Kumar,S

doi:10.3892/ijo.9.5.923

November 1996 Volume 9 Issue 5

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November 1996 Volume 9 Issue 5

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Article

Quantification of microvessel density in human tumours

Authors:
- S Abdalla
- N Haboubi
- S Kumar
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Affiliations: Affliations not specified
Pages: 923-926
|
Published online on: November 1, 1996

https://doi.org/10.3892/ijo.9.5.923
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Abstract

The current use of tumour microvascular density measurements in clinical practice is limited by the methods employed to identify and quantify the microvessels. The aim of this study was to assess tumour vascularity with three of the most commonly used endothelial markers and with different pre-treatment methods to determine which combination results in the best visualisation of endothelial cells. Serial sections of colorectal carcinoma were treated with microwave oven irradiation, pressure cooking or proteolytic digestion prior to immunostaining with von Willebrand factor (vWf), CD31 or CD34. The sensitivity of staining was enhanced by microwave pre-treatment for CD31 and CD34, and trypsinization for vWf. There was a statistically significant difference between microvascular counts obtained using antibodies for CD34 and vWf (p=0.004), and CD34 and CD31 (p=0.007), but not for CD31 and vWf (p=0.775). We conclude that the use of CD34 antibody on microwaved tissues is the most sensitive method to visualize and quantify vascular density in tumours.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Quantification of microvessel density in human tumours

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Abstract

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