8-chloro-cAMP enhances the growth inhibitory effect of cytotoxic drugs in human colon cancer cells

  • Authors:
    • G Di Isernia
    • F Ciardiello
    • C Sandomenico
    • S Pepe
    • A Bianco
    • G Tortora
  • View Affiliations

  • Published online on: December 1, 1996     https://doi.org/10.3892/ijo.9.6.1233
  • Pages: 1233-1237
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Abstract

8-Cl-cAMP is a novel agent able to inhibit the growth of a wide variety of cancer cell types in vitro and in vivo by interfering with the protein kinase A type I (PKAI), a protein directly involved in mitogenic signalling and neoplastic transformation. In a recent phase I study conducted in cancer patients we have demonstrated that 8-Cl-cAMP, at doses devoid of toxicity, may achieve plasma concentrations in a range previously shown effective for cancer cell growth inhibition. In the present study we have investigated the effect of 8-Cl-cAMP in association with cytotoxic drugs acting by different mechanisms of action on the growth of LS174T and GEO human colon cancer cells. We here demonstrate that 8-Cl-cAMP administered after the cytotoxic drugs does not interfere with their growth inhibitory effect but rather is additive with most of them. Moreover, a synergistic effect was observed when 8-Cl-cAMP was administered after cisplatin or paclitaxel. The sequence of treatment seems to be important since pretreatment with 8-Cl-cAMP interferes with the effect of the cytoxic drugs. These results demonstrate that 8-Cl-cAMP is not only able to induce cell growth inhibition when used alone but also exhibit the capacity to enhance the efficacy of different cytotoxic drugs.

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December 1996
Volume 9 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Di Isernia G, Ciardiello F, Sandomenico C, Pepe S, Bianco A and Tortora G: 8-chloro-cAMP enhances the growth inhibitory effect of cytotoxic drugs in human colon cancer cells. Int J Oncol 9: 1233-1237, 1996.
APA
Di Isernia, G., Ciardiello, F., Sandomenico, C., Pepe, S., Bianco, A., & Tortora, G. (1996). 8-chloro-cAMP enhances the growth inhibitory effect of cytotoxic drugs in human colon cancer cells. International Journal of Oncology, 9, 1233-1237. https://doi.org/10.3892/ijo.9.6.1233
MLA
Di Isernia, G., Ciardiello, F., Sandomenico, C., Pepe, S., Bianco, A., Tortora, G."8-chloro-cAMP enhances the growth inhibitory effect of cytotoxic drugs in human colon cancer cells". International Journal of Oncology 9.6 (1996): 1233-1237.
Chicago
Di Isernia, G., Ciardiello, F., Sandomenico, C., Pepe, S., Bianco, A., Tortora, G."8-chloro-cAMP enhances the growth inhibitory effect of cytotoxic drugs in human colon cancer cells". International Journal of Oncology 9, no. 6 (1996): 1233-1237. https://doi.org/10.3892/ijo.9.6.1233