Open Access

Detection of gene mutations in gastric cancer tissues using a commercial sequencing panel

  • Authors:
    • Tomoaki Ito
    • Ryo Matoba
    • Hiroshi Maekawa
    • Mutsumi Sakurada
    • Tomoyuki Kushida
    • Hajime Orita
    • Ryo Wada
    • Koichi Sato
  • View Affiliations

  • Published online on: September 24, 2019     https://doi.org/10.3892/mco.2019.1926
  • Pages: 455-460
  • Copyright: © Ito et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Predicting malignancy is important for adequate adjuvant therapy in patients with cancer. Due to cancer being a genetic disease, the detection of gene mutations could be helpful in predicting the prognosis and efficacy of drugs. Gastric cancer is the fifth most common cancer and is the third leading cause of cancer associated mortality worldwide. Mutations in genes may correlate with clinical information in patients with gastric cancer after surgery and, therefore, may be useful for predicting the prognosis of this disease. In the present study, to assess the usefulness of a commercial sequencing panel, TruSeq® Amplicon‑Cancer Panel (Illumina), using a next‑generation sequencer (Illumina MiSeq), mutation analysis of fresh as well as formalin‑fixed paraffin‑embedded (FFPE) gastric cancer tissues was performed retrospectively. The study group comprised of 4 patients who underwent gastrectomy for gastric cancer. Cancer and normal stomach tissues were collected immediately following surgical removal. Thereafter, the specimens were fixed in 10% neutral formalin for 24‑72 h. Normal and FFPE cancer tissues were histologically examined and confirmed. A total of 3 mutations were identified in the driver genes (KRAS, TP53 and APC) in cancer tissues from 2 of the 4 patients, using fresh samples. In addition, FFPE samples were analysed for the same tissues and the same results were obtained by setting the threshold for the percentage of the mutation rate to avoid detection of pseudo‑positive mutations. In conclusion, the sequencing analysis using FFPE‑derived DNA samples was successfully performed.
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November-2019
Volume 11 Issue 5

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Spandidos Publications style
Ito T, Matoba R, Maekawa H, Sakurada M, Kushida T, Orita H, Wada R and Sato K: Detection of gene mutations in gastric cancer tissues using a commercial sequencing panel. Mol Clin Oncol 11: 455-460, 2019.
APA
Ito, T., Matoba, R., Maekawa, H., Sakurada, M., Kushida, T., Orita, H. ... Sato, K. (2019). Detection of gene mutations in gastric cancer tissues using a commercial sequencing panel. Molecular and Clinical Oncology, 11, 455-460. https://doi.org/10.3892/mco.2019.1926
MLA
Ito, T., Matoba, R., Maekawa, H., Sakurada, M., Kushida, T., Orita, H., Wada, R., Sato, K."Detection of gene mutations in gastric cancer tissues using a commercial sequencing panel". Molecular and Clinical Oncology 11.5 (2019): 455-460.
Chicago
Ito, T., Matoba, R., Maekawa, H., Sakurada, M., Kushida, T., Orita, H., Wada, R., Sato, K."Detection of gene mutations in gastric cancer tissues using a commercial sequencing panel". Molecular and Clinical Oncology 11, no. 5 (2019): 455-460. https://doi.org/10.3892/mco.2019.1926