IL-10 plays an important role as an immune-modulator in the pathogenesis of atopic diseases

  • Authors:
    • Minako Kawamoto
    • Eiko Matsui
    • Hideo Kaneko
    • Toshiyuki Fukao
    • Takahide Teramoto
    • Kimiko Kasahara
    • Naomi Kondo
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/mmr_00000037
  • Pages: 837-842
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Interleukin (IL)-10 has anti-inflammatory activities in various immune reactions and plays an important role in the regulation of immune diseases. In the present study, we examined the role of IL-10 in atopic diseases. Peripheral blood mononuclear cells (PBMCs) from healthy control subjects, patients with atopic dermatitis and patients with bronchial asthma were cultured with lipopolysaccharide (LPS). The production of IL-10, IL-12 or IFN-γ by PBMCs stimulated with LPS was measured. Next, we investigated whether the haplotype in the IL-10 gene promoter region had an effect on the production of IL-10 by PBMCs. PBMCs from patients were cultured with phytohemagglutinin, to which recombinant human IL-10 had been added. IL-12, IFN-γ and IL-4 production by PBMCs was measured. β-lactoglobulin (BLG)-specific T cell clones were cultured with BLG peptide (P-17), antigen-presenting cells and recombinant human IL-10. The antigen-induced proliferation of the T cell clones and cytokine production were assayed. Results demonstrated that IL-10 production by LPS-stimulated PBMCs was lower in atopic patients than in healthy control subjects. Three different haplotypes in the IL-10 gene promoter region were detected. These haplotypes did not correlate with IL-10 production by PBMCs. IL-10 inhibited Th1 cytokine production by PBMCs, and also inhibited the antigen-induced proliferation of T cell clones and Th2 cytokine production. In conclusion, IL-10 inhibits both the production of Th1 and Th2 cytokines and the antigen-induced proliferation of T cell clones. Thus, IL-10 modulates other cytokines and plays an important role as an immune-modulator in the pathogenesis of atopic diseases.

Related Articles

Journal Cover

November 2008
Volume 1 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kawamoto, M., Matsui, E., Kaneko, H., Fukao, T., Teramoto, T., Kasahara, K., & Kondo, N. (2008). IL-10 plays an important role as an immune-modulator in the pathogenesis of atopic diseases. Molecular Medicine Reports, 1, 837-842. https://doi.org/10.3892/mmr_00000037
MLA
Kawamoto, M., Matsui, E., Kaneko, H., Fukao, T., Teramoto, T., Kasahara, K., Kondo, N."IL-10 plays an important role as an immune-modulator in the pathogenesis of atopic diseases". Molecular Medicine Reports 1.6 (2008): 837-842.
Chicago
Kawamoto, M., Matsui, E., Kaneko, H., Fukao, T., Teramoto, T., Kasahara, K., Kondo, N."IL-10 plays an important role as an immune-modulator in the pathogenesis of atopic diseases". Molecular Medicine Reports 1, no. 6 (2008): 837-842. https://doi.org/10.3892/mmr_00000037