Effects of a single-dose administration of Bowman-Birk inhibitor concentrate on anti-proliferation and inhabitation of metastasis in M5076 ovarian sarcoma-bearing mice

  • Authors:
    • Noritaka Sakurai
    • Kazuyuki Suzuki
    • Yuki Sano
    • Teruyoshi Saito
    • Hisashi Yoshimura
    • Yukie Nishimura
    • Tomohiro Yano
    • Yasuyuki Sadzuka
    • Ryuji Asano
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/mmr_00000048
  • Pages: 903-907
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Abstract

The present study was designed to investigate the effects of Bowman-Birk inhibitor concentrate (BBIC) on anti-proliferation, up-regulation of Connexin 43 (Cx43) expression and inhabitation of hepatic metastasis in mice with M5076 ovarian sarcoma. M5076 ovarian sarcomas (1x106 cells/animal) were subcutaneously transplanted into the back of BDF1 mice. The ‘pre-treated’ (n=10) and ‘post-treated’ (n=10) groups were fed a standard diet (CE-2) compounded with 0.5% BBI from 3 weeks before and from the day of tumor inoculation, respectively, until 4 weeks after tumor inoculation. The ‘control group’ (n=10) was fed CE-2 alone. Relative tumor weights in the pre- (0.013±0.010) and post- (0.012±0.013) treated groups were significantly reduced by 30.0 and 32.5%, respectively, as compared to the control group (0.040±0.022, p<0.01). The relative densities of Cx43 mRNA and Cx43 protein were significantly higher in the BBIC-treated groups than in the control group. The median numbers of macroscopic spontaneous metastases were significantly lower in the pre- (1.0±2.3) and post- (1.9±3.6) treated groups than in the control group (71.4±97.3). These results suggest that BBIC reduces proliferation as a result of increased expression of Cx43 genes in mice with M5076 ovarian sarcoma. In addition, BBIC inhibits hepatic metastasis in M5076-bearing mice.

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November 2008
Volume 1 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Sakurai, N., Suzuki, K., Sano, Y., Saito, T., Yoshimura, H., Nishimura, Y. ... Asano, R. (2008). Effects of a single-dose administration of Bowman-Birk inhibitor concentrate on anti-proliferation and inhabitation of metastasis in M5076 ovarian sarcoma-bearing mice. Molecular Medicine Reports, 1, 903-907. https://doi.org/10.3892/mmr_00000048
MLA
Sakurai, N., Suzuki, K., Sano, Y., Saito, T., Yoshimura, H., Nishimura, Y., Yano, T., Sadzuka, Y., Asano, R."Effects of a single-dose administration of Bowman-Birk inhibitor concentrate on anti-proliferation and inhabitation of metastasis in M5076 ovarian sarcoma-bearing mice". Molecular Medicine Reports 1.6 (2008): 903-907.
Chicago
Sakurai, N., Suzuki, K., Sano, Y., Saito, T., Yoshimura, H., Nishimura, Y., Yano, T., Sadzuka, Y., Asano, R."Effects of a single-dose administration of Bowman-Birk inhibitor concentrate on anti-proliferation and inhabitation of metastasis in M5076 ovarian sarcoma-bearing mice". Molecular Medicine Reports 1, no. 6 (2008): 903-907. https://doi.org/10.3892/mmr_00000048