Open Access

MMC controlled-release membranes attenuate epidural scar formation in rat models after laminectomy

  • Authors:
    • Hao Xie
    • Binbin Wang
    • Xun Shen
    • Jian Qin
    • Longhai Jiang
    • Chen Yu
    • Dawei Geng
    • Tangbo Yuan
    • Tao Wu
    • Xiaojian Cao
    • Jun Liu
  • View Affiliations

  • Published online on: April 28, 2017     https://doi.org/10.3892/mmr.2017.6531
  • Pages: 4162-4168
  • Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Epidural scar formation after laminectomy impede surgical outcomes of decompression. Mitomycin C (MMC) has been demonstrated to have significant inhibitory effects on epidural scar. This study was undertaken to develop an effective MMC controlled‑release membrane and to investigate its effects on epidural scar in rat models of laminectomy. A total of 72 rats that underwent laminectomy were divided into three groups. Among them, 24 were treated with mitomycin C‑polylactic acid (MMC-PLA) controlled‑release membrane, 24 with mitomycin C-polyethylene glycol (MMC-PEG) controlled-release membrane, and no treatment was performed for the remaining 24 rats (control group). In the following 4 weeks, magnetic resonance image (MRI), macroscopic observation, histology and hydroxyproline (Hyp) concentration analysis were performed to explore the effects of these three therapies on epidural scar. MRI revealed a significant reduction of epidural fibrosis in MMC-PLA and MMC-PEG treatment groups, compared with the control group. Histological results also showed that collagen deposition was significantly reduced after being treated with MMC-PLA or MMC-PEG membranes. Likewise, Hyp concentrations of the epidural scar tissue in MMC-PLA and MMC-PEG groups were markedly lower than those in the control group. However, regarding the effects on reducing epidural scar, no significant difference was found between the MMC-PLA and MMC-PEG groups. In conclusion, MMC-PLA and MMC-PEG membranes are safe and effective in reducing fibrosis. Thus, MMC-controlled-release membranes promises to be a potential therapeutic in preventing epidural scar formation after laminectomy.
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June-2017
Volume 15 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xie H, Wang B, Shen X, Qin J, Jiang L, Yu C, Geng D, Yuan T, Wu T, Cao X, Cao X, et al: MMC controlled-release membranes attenuate epidural scar formation in rat models after laminectomy. Mol Med Rep 15: 4162-4168, 2017
APA
Xie, H., Wang, B., Shen, X., Qin, J., Jiang, L., Yu, C. ... Liu, J. (2017). MMC controlled-release membranes attenuate epidural scar formation in rat models after laminectomy. Molecular Medicine Reports, 15, 4162-4168. https://doi.org/10.3892/mmr.2017.6531
MLA
Xie, H., Wang, B., Shen, X., Qin, J., Jiang, L., Yu, C., Geng, D., Yuan, T., Wu, T., Cao, X., Liu, J."MMC controlled-release membranes attenuate epidural scar formation in rat models after laminectomy". Molecular Medicine Reports 15.6 (2017): 4162-4168.
Chicago
Xie, H., Wang, B., Shen, X., Qin, J., Jiang, L., Yu, C., Geng, D., Yuan, T., Wu, T., Cao, X., Liu, J."MMC controlled-release membranes attenuate epidural scar formation in rat models after laminectomy". Molecular Medicine Reports 15, no. 6 (2017): 4162-4168. https://doi.org/10.3892/mmr.2017.6531