Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma

  • Authors:
    • Yonghua Fei
    • Ping Guo
    • Fuling Wang
    • Hu Li
    • Yanhua Lei
    • Wei Li
    • Xuehong Xun
    • Fengxiang Lu
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/mmr.2017.7123
  • Pages: 4179-4186
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Abstract

The aim of the present study was to elaborate the underlying pathogenesis of laryngeal squamous cell carcinoma (LSCC). Micro (mi) RNA and messenger (m) RNA expression profiling of patients with LSCC were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed miRNAs (DEMIs) and differentially expressed mRNAs (DEMs) were identified in LSCC compared to normal control tissues. The DEMs targeted by DEMIs were identified and the negative correlation between DEMs and DEMIs was subjected to visualization. The potential functions of DEMs targeted by DEMIs were annotated in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A total of 663 dysregulated DEMs (449 upregulated and 214 downregulated) and 33 DEMIs (24 upregulated and 8 downregulated) were identified in LSCC compared with normal controls. 502 negative correlations between DEMIs and DEMs were identified and subjected to construct interaction network. In the network, hsa‑miR‑486, ‑34c, ‑206 and ‑182 had the highest connectivity with DEMs, and respectively regulated 39, 33, 28 and 27 DEMs. DEMs targeted by DEMIs were significantly enriched in signal transduction, actin binding and extracellular region of GO terms and focal adhesion and extracellular matrix‑receptor interaction of KEGG pathways. The present study may provide valuable information for understanding the potential oncogenesis mechanism in LSCC and provide the foundation work for diagnosis biomarkers and therapeutic targets for LSCC.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Fei Y, Guo P, Wang F, Li H, Lei Y, Li W, Xun X and Lu F: Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma. Mol Med Rep 16: 4179-4186, 2017.
APA
Fei, Y., Guo, P., Wang, F., Li, H., Lei, Y., Li, W. ... Lu, F. (2017). Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma. Molecular Medicine Reports, 16, 4179-4186. https://doi.org/10.3892/mmr.2017.7123
MLA
Fei, Y., Guo, P., Wang, F., Li, H., Lei, Y., Li, W., Xun, X., Lu, F."Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma". Molecular Medicine Reports 16.4 (2017): 4179-4186.
Chicago
Fei, Y., Guo, P., Wang, F., Li, H., Lei, Y., Li, W., Xun, X., Lu, F."Identification of miRNA-mRNA crosstalk in laryngeal squamous cell carcinoma". Molecular Medicine Reports 16, no. 4 (2017): 4179-4186. https://doi.org/10.3892/mmr.2017.7123