Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro

  • Authors:
    • Lihui Ren
    • Ping Wang
    • Zuoyan Wang
    • Yong Liu
    • Shuzheng Lv
  • View Affiliations

  • Published online on: July 21, 2017     https://doi.org/10.3892/mmr.2017.7066
  • Pages: 4334-4340
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the current study was to investigate the cytotoxic effects of hypotonic (iopamidol) and isotonic (iodixanol) contract media (CMs) in vitro and in vivo. A total of 60 Wistar rats were included and were randomly divided into three groups (20 rats per group). Iodixanol (4 g iodine/kg), iopamidol (4 g iodine/kg) or equal volume of normal saline was injected via tail vein. HUVEC and H5V cell viability was determined by Cell Counting Kit‑8 agents. Western blotting was performed to detect ATP‑binding cassette subfamily G member 1 (ABCG1) expression. For histological analysis, hematoxylin and eosin staining was performed. Plasma endothelin, von Willebrand factor, tissue type plasminogen activator, plasminogen activator inhibitor, D‑Dimer, fibrinogen, anti‑thrombin III, plasminogen and nitric oxide synthase (NOS) were measured by using ELISA. Both iopamidol and iodixanol treatments deceased cell viability and increased apoptosis of HUVEC and H5V cells, along with downregulated NOS and ABCG1. The injection of iopamidol or iodixanol into rats changed the endothelium‑related plasma levels of biomarkers, including endothelin, von Willebrand factor, tissue type plasminogen activator, plasminogen activator inhibitor, D‑Dimer, fibrinogen and anti‑thrombin III. However, endothelia isolated from rat abdominal aorta in the iodixanol group retained their normal structure, whereas endothelial structure in the iopamidol group was injured and disrupted. The findings in the present study suggested that both hypotonic and isotonic CMs may lead to endothelial dysfunction and thrombin and fibrinolytic system disorder. However, hypotonic CMs may be more toxic than isotonic CMs. Therefore, additional cautions should be taken when selecting hypotonic CMs and their dosages during cardioangiography.

Related Articles

Journal Cover

October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ren L, Wang P, Wang Z, Liu Y and Lv S: Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro. Mol Med Rep 16: 4334-4340, 2017
APA
Ren, L., Wang, P., Wang, Z., Liu, Y., & Lv, S. (2017). Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro. Molecular Medicine Reports, 16, 4334-4340. https://doi.org/10.3892/mmr.2017.7066
MLA
Ren, L., Wang, P., Wang, Z., Liu, Y., Lv, S."Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro". Molecular Medicine Reports 16.4 (2017): 4334-4340.
Chicago
Ren, L., Wang, P., Wang, Z., Liu, Y., Lv, S."Hypotonic contrast media is more toxic than isotonic contrast media on endothelial cells in vivo and in vitro". Molecular Medicine Reports 16, no. 4 (2017): 4334-4340. https://doi.org/10.3892/mmr.2017.7066