Open Access

Saponin extract from Panax notoginseng promotesangiogenesis through AMPK‑ and eNOS‑dependent pathways in HUVECs

  • Authors:
    • Dongzhi Wang
    • Qiqiang Jie
    • Baoxin Liu
    • Yong Li
    • Liming Dai
    • Jiachen Luo
    • Lei Hou
    • Yidong Wei
  • View Affiliations

  • Published online on: August 18, 2017     https://doi.org/10.3892/mmr.2017.7280
  • Pages: 5211-5218
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Panax notoginseng saponins (PNS) are among the most important compounds extracted from Panax notoginseng root, and have long been used in traditional Chinese medicine to control bleeding. PNS have recently garnered attention for the treatment of circulatory system diseases. The present study aimed to evaluate the effects of PNS on angiogenesis in vitro and to explore the molecular mechanisms underlying their actions. The present results demonstrated that the proliferative ability of human umbilical vein endothelial cells (HUVECs) was augmented following treatment with PNS. In addition, wound healing and Boyden chamber assays indicated that PNS may enhance HUVEC motility and increase the number of capillary‑like tube branches in HUVECs. These effects were suppressed by 5' adenosine monophosphate‑activated protein kinase (AMPK) and endothelial nitric oxide synthase (eNOS) inhibitors. Furthermore, western blot analysis demonstrated that PNS stimulated the phosphorylation of AMPK and eNOS at Thr‑172 and Ser‑1179, respectively. These results suggested that PNS may promote tube formation in endothelial cells through AMPK‑ and eNOS‑dependent signaling pathways.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang D, Jie Q, Liu B, Li Y, Dai L, Luo J, Hou L and Wei Y: Saponin extract from Panax notoginseng promotesangiogenesis through AMPK‑ and eNOS‑dependent pathways in HUVECs. Mol Med Rep 16: 5211-5218, 2017.
APA
Wang, D., Jie, Q., Liu, B., Li, Y., Dai, L., Luo, J. ... Wei, Y. (2017). Saponin extract from Panax notoginseng promotesangiogenesis through AMPK‑ and eNOS‑dependent pathways in HUVECs. Molecular Medicine Reports, 16, 5211-5218. https://doi.org/10.3892/mmr.2017.7280
MLA
Wang, D., Jie, Q., Liu, B., Li, Y., Dai, L., Luo, J., Hou, L., Wei, Y."Saponin extract from Panax notoginseng promotesangiogenesis through AMPK‑ and eNOS‑dependent pathways in HUVECs". Molecular Medicine Reports 16.4 (2017): 5211-5218.
Chicago
Wang, D., Jie, Q., Liu, B., Li, Y., Dai, L., Luo, J., Hou, L., Wei, Y."Saponin extract from Panax notoginseng promotesangiogenesis through AMPK‑ and eNOS‑dependent pathways in HUVECs". Molecular Medicine Reports 16, no. 4 (2017): 5211-5218. https://doi.org/10.3892/mmr.2017.7280