Expression of carbonic anhydrase III and skeletal muscle remodeling following selective denervation

Huang,He; Zhao,Yanling; Shang,Xiliang; Liu,Xueyuan; Ren,Huimin

doi:10.3892/mmr.2017.7644

Expression of carbonic anhydrase III and skeletal muscle remodeling following selective denervation

Authors:
- He Huang
- Yanling Zhao
- Xiliang Shang
- Xueyuan Liu
- Huimin Ren
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Affiliations: Department of Neurology, Tenth People's Hospital of Tongji University, Shanghai 200072, P.R. China, Department of Geriatrics, Shanghai First People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200080, P.R. China, Department of Sports Medicine, Fudan University, Shanghai 200040, P.R. China, Institute of Neurology, Fudan University, Shanghai 200040, P.R. China
Published online on: September 27, 2017 https://doi.org/10.3892/mmr.2017.7644
Pages: 8289-8294

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Abstract

Carbonic anhydrase III (CAIII) is expressed selectively in type I (slow‑twitch) myofibers. To investigate the association between changes in the expression of CAIII and skeletal muscle structure following denervation, the present study stained adjacent sections of skeletal muscle for ATPase and immunohistochemically for CAIII. In addition, differences in the protein expression and phosphatase activity of CAIII were examined by western blot and phosphatase staining between rat soleus and extensol digitorum longus (EDL) muscles, which are composed of predominantly slow‑ and fast‑twitch fibers, respectively. Upon denervation, the EDL muscle showed more pronounced structural changes, compared with the soleus muscle. There was a transformation from fast to slow fibers, and a concomitant increase in fibers positive for CAIII. Following denervation, the protein expression of CAIII initially increased and then decreased in the soleus muscle, whereas the protein expression of CAIII in the EDL muscle increased gradually with time. In contrast to the protein changes, phosphatase activity in the soleus and EDL muscles decreased significantly following denervation. These results indicated that, following denervation, changes in the expression of CAIII were associated with myofiber remodeling. Specifically, the change in the expression of CAIII reflected the conversion to type I myofibers, suggesting the importance of CAIII in resistance to fatigue in skeletal muscle.

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