Open Access

Prognostic implications and procoagulant activity of phosphatidylserine exposure of blood cells and microparticles in patients with atrial fibrillation treated with pulmonary vein isolation

  • Authors:
    • Huan Meng
    • Junjie Kou
    • Ruishuang Ma
    • Wenbo Ding
    • Yan Kou
    • Muhua Cao
    • Zengxiang Dong
    • Yayan Bi
    • Hemant S. Thatte
    • Jialan Shi
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/mmr.2017.7763
  • Pages:8579-8588
  • Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to evaluate the procoagulant effects of phosphatidylserine (PS) exposure on blood cells and microparticles (MPs), and examine its role in predicting early recurrence atrial fibrillation (ERAF) in patients with atrial fibrillation (AF) treated with pulmonary vein isolation (PVI). Blood samples were obtained from 40 healthy controls and 56 patients with AF at baseline (prior to PVI), and 0, 1 h, 1 day, 3 days and 7 days following PVI. The exposure of PS (PS+) to blood cells (platelets, erythrocytes and leukocytes) and MPs was detected using flow cytometry. The procoagulant activity was evaluated by coagulation time, and the formation of factor Xa (FXa) and thrombin. In addition, independent factors associated with PS+ blood cells and MPs, and significant predictors of ERAF following PVI were investigated by statistical analyses. The numbers of PS+ blood cells and MPs were significantly increased by PVI (P<0.01). A significant decrease in coagulation time, and increases in FXa and thrombin were exhibited in the PS+ blood cells and MPs from patients with AF treated with PVI, whereas these alterations were inhibited by either lactadherin or anti‑tissue factor (P<0.01). The maximum power of the PVI was significantly associated with platelet‑derived MPs, and high‑sensitivity C‑reactive protein (hs‑CRP) was closely associated with leukocyte‑derived MPs and endothelial‑derived MPs (EMPs) (P<0.01). In addition, hs‑CRP and EMPs >355/µl were identified as independent predictors of ERAF (P<0.05). The increased numbers of PS+ platelets, erythrocytes, leukocytes and MPs contributed to the procoagulant state of AF, and hs‑CRP and EMPs were able to predict ERAF following PVI.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Meng, H., Kou, J., Ma, R., Ding, W., Kou, Y., Cao, M. ... Shi, J. (2017). Prognostic implications and procoagulant activity of phosphatidylserine exposure of blood cells and microparticles in patients with atrial fibrillation treated with pulmonary vein isolation. Molecular Medicine Reports, 16, 8579-8588. https://doi.org/10.3892/mmr.2017.7763
MLA
Meng, H., Kou, J., Ma, R., Ding, W., Kou, Y., Cao, M., Dong, Z., Bi, Y., Thatte, H. S., Shi, J."Prognostic implications and procoagulant activity of phosphatidylserine exposure of blood cells and microparticles in patients with atrial fibrillation treated with pulmonary vein isolation". Molecular Medicine Reports 16.6 (2017): 8579-8588.
Chicago
Meng, H., Kou, J., Ma, R., Ding, W., Kou, Y., Cao, M., Dong, Z., Bi, Y., Thatte, H. S., Shi, J."Prognostic implications and procoagulant activity of phosphatidylserine exposure of blood cells and microparticles in patients with atrial fibrillation treated with pulmonary vein isolation". Molecular Medicine Reports 16, no. 6 (2017): 8579-8588. https://doi.org/10.3892/mmr.2017.7763