Open Access

MACC1 facilitates chemoresistance and cancer stem cell‑like properties of colon cancer cells through the PI3K/AKT signaling pathway

  • Authors:
    • Jiankai Wang
    • Wenjuan Wang
    • Hongyi Cai
    • Binbin Du
    • Lijuan Zhang
    • Wen Ma
    • Yongguo Hu
    • Shifang Feng
    • Guoying Miao
  • View Affiliations

  • Published online on: October 4, 2017     https://doi.org/10.3892/mmr.2017.7721
  • Pages:8747-8754
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

With regards to colon cancer, resistance to 5‑fluorouracil (5‑FU)‑based chemotherapy and cancer stem cells (CSCs) are considered important factors underlying therapy failure. Metastasis‑associated colon cancer 1 (MACC1) has been associated with poor prognosis and the promotion of metastasis within several types of cancer. However, the biological behavior of MACC1 in chemoresistance and CSC‑like properties remains unclear. In the present study, various methods including gene knockdown, gene overexpression, western blotting, quantitative polymerase chain reaction and MTT assay, have been adopted. According to the results of the present study, MACC1 was depleted in two colon cancer cell lines resistant to 5‑FU; subsequently, CSC‑like properties and 5‑FU sensitivity were investigated. Within 5‑FU‑resistant cells, cell death was facilitated by MACC1 knockdown. Furthermore, sphere formation and the expression levels of pluripotent markers, including cluster of differentiation (CD) 44, CD133 and Nanog were reduced due to MACC1 depletion. Additionally, it was indicated that the phosphoinositide 3‑kinase/protein kinase B signaling pathway may be associated with 5‑FU resistance and CSC‑like properties via MACC1.

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December 2017
Volume 16 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Wang, J., Wang, W., Cai, H., Du, B., Zhang, L., Ma, W. ... Miao, G. (2017). MACC1 facilitates chemoresistance and cancer stem cell‑like properties of colon cancer cells through the PI3K/AKT signaling pathway. Molecular Medicine Reports, 16, 8747-8754. https://doi.org/10.3892/mmr.2017.7721
MLA
Wang, J., Wang, W., Cai, H., Du, B., Zhang, L., Ma, W., Hu, Y., Feng, S., Miao, G."MACC1 facilitates chemoresistance and cancer stem cell‑like properties of colon cancer cells through the PI3K/AKT signaling pathway". Molecular Medicine Reports 16.6 (2017): 8747-8754.
Chicago
Wang, J., Wang, W., Cai, H., Du, B., Zhang, L., Ma, W., Hu, Y., Feng, S., Miao, G."MACC1 facilitates chemoresistance and cancer stem cell‑like properties of colon cancer cells through the PI3K/AKT signaling pathway". Molecular Medicine Reports 16, no. 6 (2017): 8747-8754. https://doi.org/10.3892/mmr.2017.7721